Biomaterial Database

Insulin-like immunoreactivity in serum and pancreas of metamorphosing tadpoles. 1985

J Hulsebus, and E S Farrar

Insulin-like immunoreactivity (ILI) in pancreatic extracts and serum of Rana catesbeiana tadpoles of various developmental stages was determined by radioimmunoassay. Serum ILI levels increased from 2.4 +/- 0.4 microU/ml in limb bud stage tadpoles (I-V) to 13.9 +/- 2.2 microU/ml in foot stage animals (XI-XVII). During climax, serum ILI levels declined reaching 5.0 microU/ml in postmetamorphic juveniles. Embryonic pancreases (stage 25) contained sizeable amounts of ILI (115 +/- 14.4 microU). A fourfold increase in total pancreatic ILI occurred during the limb bud stages, and a twelvefold increase occurred by the end of the foot development stages. Total pancreatic ILI declined throughout climax stages to 410 microU in postmetamorphic juveniles. Serum glucose increased slightly over the course of larval development; the peak concentration occurred in foot stage tadpoles.

UI	MeSH Term	Description	Entries
D007328	Insulin	A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).	Iletin,Insulin A Chain,Insulin B Chain,Insulin, Regular,Novolin,Sodium Insulin,Soluble Insulin,Chain, Insulin B,Insulin, Sodium,Insulin, Soluble,Regular Insulin
D008675	Metamorphosis, Biological	Profound physical changes during maturation of living organisms from the immature forms to the adult forms, such as from TADPOLES to frogs; caterpillars to BUTTERFLIES.	Biological Metamorphosis,Biological Metamorphoses,Metamorphoses, Biological
D009929	Organ Size	The measurement of an organ in volume, mass, or heaviness.	Organ Volume,Organ Weight,Size, Organ,Weight, Organ
D010179	Pancreas	A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.
D011892	Rana catesbeiana	A species of the family Ranidae (true frogs). The only anuran properly referred to by the common name "bullfrog", it is the largest native anuran in North America.	Bullfrog,Bullfrogs,Rana catesbeianas,catesbeiana, Rana
D001786	Blood Glucose	Glucose in blood.	Blood Sugar,Glucose, Blood,Sugar, Blood
D001835	Body Weight	The mass or quantity of heaviness of an individual. It is expressed by units of pounds or kilograms.	Body Weights,Weight, Body,Weights, Body
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia