-transparent.png){kind=logo}
A rat monoclonal antibody, LO SM2, of the immunoglobulin M class, specific for a saliva receptor (SR) from Streptococcus mutans serotype f, was able to precipitate the SR from crude cell-wall-associated antigens (WEA) of this bacteria in presence of a detergent mixture. We have then used the technique of monoclonal-antibody immunoaffinity chromatography to purify the S. mutans SR. Pure SR was obtained from a crude WEA fraction with a single chromatographic step. The active SR could be eluted from the column in a highly purified form with 0.2 M-glycine/HC1, pH 2.8. The final yield was about 32% in terms of binding activity. Characterization of the SR by crossed immunoelectrophoresis, sodium dodecyl sulphate- or 4-30%-native-gradient-polyacrylamide-gel electrophoresis showed that the receptor is a single polypeptide chain of Mr approx. 74000. Native or denaturated forms of the SR adsorbed on to a solid support, such as nitrocellulose, are recognized by monoclonal antibody LO SM2, and both forms are still able to bind the ligand, saliva.
F Ackermans, and
J P Klein, and
J Ogier, and
H Bazin, and
F Cormont, and
R M Frank
September 1990,
Kisaengch'unghak chapchi. The Korean journal of parasitology,
F Ackermans, and
J P Klein, and
J Ogier, and
H Bazin, and
F Cormont, and
R M Frank
January 1995,
Methods in molecular biology (Clifton, N.J.),
F Ackermans, and
J P Klein, and
J Ogier, and
H Bazin, and
F Cormont, and
R M Frank
June 1990,
Journal of biochemical and biophysical methods,
F Ackermans, and
J P Klein, and
J Ogier, and
H Bazin, and
F Cormont, and
R M Frank
January 1980,
Infection and immunity,
F Ackermans, and
J P Klein, and
J Ogier, and
H Bazin, and
F Cormont, and
R M Frank
April 1987,
Biochemistry and cell biology = Biochimie et biologie cellulaire,
F Ackermans, and
J P Klein, and
J Ogier, and
H Bazin, and
F Cormont, and
R M Frank
November 1987,
Journal of bacteriology,
F Ackermans, and
J P Klein, and
J Ogier, and
H Bazin, and
F Cormont, and
R M Frank
May 1987,
Journal of general microbiology,
F Ackermans, and
J P Klein, and
J Ogier, and
H Bazin, and
F Cormont, and
R M Frank
October 1982,
Biochimica et biophysica acta,
F Ackermans, and
J P Klein, and
J Ogier, and
H Bazin, and
F Cormont, and
R M Frank
February 1985,
Biochemical and biophysical research communications,
F Ackermans, and
J P Klein, and
J Ogier, and
H Bazin, and
F Cormont, and
R M Frank
February 1992,
Canadian journal of microbiology,
