-transparent.png){kind=logo}
In these competitive and "sandwich"-type heterogeneous enzyme immunoassays, based on liquid chromatography with electrochemical detection, rabbit immunoglobulin G is used as a model compound. Alkaline phosphatase (EC 3.1.3.1), the labeling enzyme, catalyzes conversion of phenyl phosphate to phenol. After separation on an octyldecylsilane column, the enzyme-generated phenol is detected in a thin-layer cell at a carbon-paste working electrode. The detection limit for phenol is 5.0 nmol/L. The electrode response varies linearly with concentration over a range of three orders of magnitude. For the sandwich-type assay procedure the detection limit is 10 ng/L; the linearity ranges over four orders of magnitude. The detection limit of the competitive immunoassay is 5 micrograms/L. The dynamic range spans two orders of magnitude.
K R Wehmeyer, and
H B Halsall, and
W R Heineman
January 1990,
Immunology series,
K R Wehmeyer, and
H B Halsall, and
W R Heineman
April 1990,
Clinical chemistry,
K R Wehmeyer, and
H B Halsall, and
W R Heineman
June 2023,
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry,
K R Wehmeyer, and
H B Halsall, and
W R Heineman
January 1990,
Journal of immunoassay,
K R Wehmeyer, and
H B Halsall, and
W R Heineman
May 2000,
Clinical and diagnostic laboratory immunology,
K R Wehmeyer, and
H B Halsall, and
W R Heineman
January 1987,
Zeitschrift fur medizinische Laboratoriumsdiagnostik,
K R Wehmeyer, and
H B Halsall, and
W R Heineman
September 1973,
Clinica chimica acta; international journal of clinical chemistry,
K R Wehmeyer, and
H B Halsall, and
W R Heineman
August 2018,
Talanta,
K R Wehmeyer, and
H B Halsall, and
W R Heineman
June 1985,
Journal of immunological methods,
K R Wehmeyer, and
H B Halsall, and
W R Heineman
December 1987,
Analytical chemistry,
