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The mechanism of organic anion uptake by hepatocytes has kinetics that suggest facilitated diffusion, and carrier-mediated membrane transport has been postulated. In previous studies, we purified a 55,000-mol wt organic anion-binding protein (OABP) by affinity chromatography on sulfobromophthalein (BSP)-Sepharose of deoxycholate solubilized liver cell plasma membrane preparations. Using specific goat and rabbit antibodies to OABP, we have now investigated the distribution of this protein in liver fractions and other tissues by an enzyme-linked immunosorbent assay and by the immunoblot (Western blot) procedure. These studies indicated that OABP is present in significant amounts in all tissues examined except for blood. Although OABP has not as yet been isolated from each of these tissues and characterized, OABP in heart retained the ability to bind organic anions, and was purified by affinity chromatography on BSP-sepharose. In liver, OABP was membrane bound and remained so after extraction with 0.9 M NaCl, which suggests that it is an intrinsic membrane protein. OABP did not have a ubiquitous subcellular distribution within the hepatocyte. Preparation of subfractions of liver cell plasma membrane revealed that OABP is present in the sinusoidal and absent from the canalicular membrane. Immunofluorescence studies performed in short-term cultured hepatocytes suggest that OABP is associated with the surface of these cells and does not have a significant intracellular distribution.
A W Wolkoff, and
A Sosiak, and
H C Greenblatt, and
J Van Renswoude, and
R J Stockert
May 1980,
The Journal of clinical investigation,
A W Wolkoff, and
A Sosiak, and
H C Greenblatt, and
J Van Renswoude, and
R J Stockert
November 1979,
Biochemical and biophysical research communications,
A W Wolkoff, and
A Sosiak, and
H C Greenblatt, and
J Van Renswoude, and
R J Stockert
March 1972,
The Journal of clinical investigation,
A W Wolkoff, and
A Sosiak, and
H C Greenblatt, and
J Van Renswoude, and
R J Stockert
January 1981,
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A W Wolkoff, and
A Sosiak, and
H C Greenblatt, and
J Van Renswoude, and
R J Stockert
January 1980,
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A W Wolkoff, and
A Sosiak, and
H C Greenblatt, and
J Van Renswoude, and
R J Stockert
October 1993,
Biochemistry and molecular biology international,
A W Wolkoff, and
A Sosiak, and
H C Greenblatt, and
J Van Renswoude, and
R J Stockert
March 1991,
The Journal of membrane biology,
A W Wolkoff, and
A Sosiak, and
H C Greenblatt, and
J Van Renswoude, and
R J Stockert
September 1969,
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A W Wolkoff, and
A Sosiak, and
H C Greenblatt, and
J Van Renswoude, and
R J Stockert
February 1982,
Biochimica et biophysica acta,
A W Wolkoff, and
A Sosiak, and
H C Greenblatt, and
J Van Renswoude, and
R J Stockert
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