-transparent.png){kind=logo}
Essential replication (rep) genes of the broad host range plasmid RSF1010 have been cloned onto controlled expression vectors and their protein products have been visualized, after induction, by NaDodSO4/polyacrylamide gel electrophoresis of whole cell lysates. During this induction the replication of a coresident RSF1010 replicon, pKT210, was analyzed by quantitative DNA X DNA hybridization. The initiation of pKT210 replication was stimulated 6-fold by a simultaneous overproduction of the RepA and RepC proteins compared to cells in which only the RepA protein was overproduced. An enhanced synthesis of the RepB protein resulted in a 1.6-fold stimulation of pKT210 replication, whereas an overproduction of the RepA protein alone had no effect. Purified RepC protein has been shown to bind preferentially to DNA carrying the replication origin of RSF1010. Within this segment it was bound specifically to those DNA fragments that contained the 20-base-pair direct repeats of the origin region. These results suggest that RepC protein acts as a positive replication regulator, that its concentration is rate-limiting, and that the replication rate of RSF1010 is controlled, at least in part, at the level of RepC synthesis.
V Haring, and
P Scholz, and
E Scherzinger, and
J Frey, and
K Derbyshire, and
G Hatfull, and
N S Willetts, and
M Bagdasarian
April 1992,
Gene,
V Haring, and
P Scholz, and
E Scherzinger, and
J Frey, and
K Derbyshire, and
G Hatfull, and
N S Willetts, and
M Bagdasarian
August 1995,
Nucleic acids research,
V Haring, and
P Scholz, and
E Scherzinger, and
J Frey, and
K Derbyshire, and
G Hatfull, and
N S Willetts, and
M Bagdasarian
January 1985,
Basic life sciences,
V Haring, and
P Scholz, and
E Scherzinger, and
J Frey, and
K Derbyshire, and
G Hatfull, and
N S Willetts, and
M Bagdasarian
January 1986,
Plasmid,
V Haring, and
P Scholz, and
E Scherzinger, and
J Frey, and
K Derbyshire, and
G Hatfull, and
N S Willetts, and
M Bagdasarian
May 1982,
Journal of bacteriology,
V Haring, and
P Scholz, and
E Scherzinger, and
J Frey, and
K Derbyshire, and
G Hatfull, and
N S Willetts, and
M Bagdasarian
November 1986,
Plasmid,
V Haring, and
P Scholz, and
E Scherzinger, and
J Frey, and
K Derbyshire, and
G Hatfull, and
N S Willetts, and
M Bagdasarian
October 1986,
Nucleic acids research,
V Haring, and
P Scholz, and
E Scherzinger, and
J Frey, and
K Derbyshire, and
G Hatfull, and
N S Willetts, and
M Bagdasarian
February 1984,
Proceedings of the National Academy of Sciences of the United States of America,
V Haring, and
P Scholz, and
E Scherzinger, and
J Frey, and
K Derbyshire, and
G Hatfull, and
N S Willetts, and
M Bagdasarian
October 1985,
Journal of molecular biology,
V Haring, and
P Scholz, and
E Scherzinger, and
J Frey, and
K Derbyshire, and
G Hatfull, and
N S Willetts, and
M Bagdasarian
November 1997,
Applied and environmental microbiology,
