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A quantitative assay of IgA class circulating immune complexes (IgA-CIC) by a solid phase anti-C3 enzyme immunoassay (anti-C3 EIA) is described. A stable and reproducible standard for determination of IgA-CIC was prepared successfully by chemical binding of complement C3 to human serum IgA. Two of 27 sera from patients with systemic lupus erythematosus (SLE), however, contained high concentrations of IgA class anti-F(ab')2 antibodies that caused false positive results when the F(ab')2 of anti-C3 was used for EIA. Solid phase Facb of anti-C3 was found to eliminate the false positive results caused by IgA class anti-F(ab')2 and IgA class rheumatoid factor. Good reproducibility and recovery were observed with this Facb anti-C3 EIA using the IgA-C3, a stable standard material, and so this method should be useful clinically in elucidating the role of IgA-CIC.
K Hosoda, and
I Sakurabayashi, and
T Kawai, and
H Suzuki, and
M Maeda, and
A Tsuji
February 1987,
Clinical and experimental immunology,
K Hosoda, and
I Sakurabayashi, and
T Kawai, and
H Suzuki, and
M Maeda, and
A Tsuji
February 1988,
The Tokai journal of experimental and clinical medicine,
K Hosoda, and
I Sakurabayashi, and
T Kawai, and
H Suzuki, and
M Maeda, and
A Tsuji
August 1980,
Journal of immunology (Baltimore, Md. : 1950),
K Hosoda, and
I Sakurabayashi, and
T Kawai, and
H Suzuki, and
M Maeda, and
A Tsuji
January 1983,
Biomedica biochimica acta,
K Hosoda, and
I Sakurabayashi, and
T Kawai, and
H Suzuki, and
M Maeda, and
A Tsuji
March 1982,
Journal of clinical microbiology,
K Hosoda, and
I Sakurabayashi, and
T Kawai, and
H Suzuki, and
M Maeda, and
A Tsuji
February 1984,
Arerugi = [Allergy],
K Hosoda, and
I Sakurabayashi, and
T Kawai, and
H Suzuki, and
M Maeda, and
A Tsuji
October 1983,
Journal of immunological methods,
K Hosoda, and
I Sakurabayashi, and
T Kawai, and
H Suzuki, and
M Maeda, and
A Tsuji
December 1986,
Rinsho byori. The Japanese journal of clinical pathology,
K Hosoda, and
I Sakurabayashi, and
T Kawai, and
H Suzuki, and
M Maeda, and
A Tsuji
December 1987,
Rinsho byori. The Japanese journal of clinical pathology,
K Hosoda, and
I Sakurabayashi, and
T Kawai, and
H Suzuki, and
M Maeda, and
A Tsuji
April 1984,
Journal of immunological methods,
