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The pre- and postnatal development of the adrenal medulla was examined in the rat by immunohistochemistry and by assay of catecholamines. Immunohistochemistry involved the use of antibodies to noradrenaline (NA), adrenaline (A) and the biosynthesizing enzymes dopamine beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT). Adrenal glands were obtained from animals from the 16th day of gestation to the 7th postnatal day at daily intervals, and at the 14th postnatal day, and from adult rats. Tissues were fixed in ice-cold, 4% paraformaldehyde, buffered at pH 7.3. Cryostat sections (7 microns) were stained with the indirect immunofluorescence technique. Adrenals from the same developmental stages were assayed for the presence of DA (dopamine), NA and A by ion-pair reversed-phase liquid chromatography with electrochemical detection. In adult adrenals the majority of the medullary cells (approximately 80%) were highly immunoreactive to A and moderately immunoreactive to NA. They also showed immunoreactivity to both DBH and PNMT, i.e., they are synthesizing and storing A. The remaining cell clusters were only stained by antibodies to DBH and NA (NA-synthesizing and -storing cells). These findings correlate well with the relative concentrations of A and NA as determined by assay. Three developmental phases could be distinguished. In the first phase, the 16th and 17th prenatal day, medullary cells were only immunoreactive to DBH and NA, and only very small amounts of A as compared to NA were found. During the second period, from the 18th prenatal day to 2 or 3 days after birth, all medullary cells were immunoreactive to DBH, NA, PNMT and A, and during this phase the adrenaline concentration increased daily and became the predominant amine on the 20th day of gestation. Adrenaline represented 75% of total catecholamine on the 1st to 3rd day after birth. The third phase started at the 2nd or 3rd postnatal day and was characterized by the presence of an increasing number of medullary cells solely immunoreactive to DBH and NA, hence synthesizing and storing NA. The remaining cells were immunoreactive to DBH, NA, PNMT and A. Postnatally, the relative concentration of A continued to rise reaching 79% by the 4th postnatal day. These results indicate that initially the adrenal medullary cells are synthesizing and storing almost exclusively NA.(ABSTRACT TRUNCATED AT 400 WORDS)
A A Verhofstad, and
R E Coupland, and
T R Parker, and
M Goldstein
January 1961,
Naunyn-Schmiedebergs Archiv fur experimentelle Pathologie und Pharmakologie,
A A Verhofstad, and
R E Coupland, and
T R Parker, and
M Goldstein
January 1992,
Medicinski pregled,
A A Verhofstad, and
R E Coupland, and
T R Parker, and
M Goldstein
April 1972,
Archives internationales de pharmacodynamie et de therapie,
A A Verhofstad, and
R E Coupland, and
T R Parker, and
M Goldstein
June 1984,
The Journal of endocrinology,
A A Verhofstad, and
R E Coupland, and
T R Parker, and
M Goldstein
June 1957,
Endocrinology,
A A Verhofstad, and
R E Coupland, and
T R Parker, and
M Goldstein
August 1994,
Neuroscience letters,
A A Verhofstad, and
R E Coupland, and
T R Parker, and
M Goldstein
January 1965,
Acta biologica Academiae Scientiarum Hungaricae,
A A Verhofstad, and
R E Coupland, and
T R Parker, and
M Goldstein
January 1955,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
A A Verhofstad, and
R E Coupland, and
T R Parker, and
M Goldstein
March 1954,
The Journal of physiology,
A A Verhofstad, and
R E Coupland, and
T R Parker, and
M Goldstein
March 1969,
Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles,
