Biomaterial Database

Identification of proline carrier in Escherichia coli K-12. 1985

K Hanada, and I Yamato, and Y Anraku

Proline carrier, a product of the putP gene of Escherichia coli, was identified as a 35 kDa cytoplasmic membrane protein by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Its identification was based on the following evidence: First, the density of the band corresponding to a 35 kDa protein correlated with the proline-binding activity of cytoplasmic membranes from putP-deficient and putP-amplified strains. Second, by the differential labeling method, the 35 kDa protein was specifically labeled with radioactive N-ethyl-maleimide. The 35 kDa protein was found to aggregate on heat treatment and to show abnormal mobility on SDS-PAGE.

UI	MeSH Term	Description	Entries
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D011392	Proline	A non-essential amino acid that is synthesized from GLUTAMIC ACID. It is an essential component of COLLAGEN and is important for proper functioning of joints and tendons.	L-Proline,L Proline
D002352	Carrier Proteins	Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes.	Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D004591	Electrophoresis, Polyacrylamide Gel	Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.	Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005033	Ethylmaleimide	A sulfhydryl reagent that is widely used in experimental biochemical studies.	N-Ethylmaleimide,N Ethylmaleimide
D046911	Macromolecular Substances	Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.	Macromolecular Complexes,Macromolecular Compounds,Macromolecular Compounds and Complexes,Complexes, Macromolecular,Compounds, Macromolecular,Substances, Macromolecular
D026921	Amino Acid Transport Systems, Neutral	Amino acid transporter systems capable of transporting neutral amino acids (AMINO ACIDS, NEUTRAL).	Neutral Amino Acid Transport Systems,Neutral Amino Acid Transporters,Zwitterionic Amino Acid Transport Systems,Na+-Independent Neutral Amino Acid Transporter,Neutral Amino Acid Transport Proteins,Sodium Dependent Neutral Amino Acid Transport Proteins,Sodium Dependent Neutral Amino Acid Transporters,Sodium Independent Neutral Amino Acid Transport Proteins,Sodium Independent Neutral Amino Acid Transporters,Zwitterionic Amino Acid Transport Proteins,Zwitterionic Amino Acid Transporters,Na+ Independent Neutral Amino Acid Transporter
D027981	Symporters	Membrane transporters that co-transport two or more dissimilar molecules in the same direction across a membrane. Usually the transport of one ion or molecule is against its electrochemical gradient and is "powered" by the movement of another ion or molecule with its electrochemical gradient.	Co-Transporter,Co-Transporters,Symporter,Co Transporter,Co Transporters