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An immunohistochemical study was carried out on a human breast carcinoma series with the aim to delineate the technical approach to distinguish the epithelial cells from stromal nonepithelial cells in the primary tumor and in cultured tumor cells. Frozen sections from 30 unfixed breast carcinomas were incubated with mouse monoclonal antiepithelial cell membrane antibodies and then with a biotinilizated antimouse antibody and the avidin biotin peroxidase complex (ABC). Thick (100 micron) sections from fresh, unfixed tissue were similarly treated prior to the araldite embedding procedure for EM study. In all cases the epithelial tumor cells were immunostained in contrast to nonepithelial stromal cells, particularly the fibroblasts. Because standard light microscopy fails to determine the real nature of the fusiforme cultured cells, it is suggested that these techniques represent a reliable method for mammary epithelial cell identification and may further be applied on cultured tissue.
C Charpin, and
P M Martin, and
J C Lissitzky, and
M N Lavaut, and
F Kopp, and
N Pourreau-Schneider, and
J Jacquemier, and
M Toga
January 1984,
Acta histochemica. Supplementband,
C Charpin, and
P M Martin, and
J C Lissitzky, and
M N Lavaut, and
F Kopp, and
N Pourreau-Schneider, and
J Jacquemier, and
M Toga
August 1980,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
C Charpin, and
P M Martin, and
J C Lissitzky, and
M N Lavaut, and
F Kopp, and
N Pourreau-Schneider, and
J Jacquemier, and
M Toga
August 1987,
American journal of clinical pathology,
C Charpin, and
P M Martin, and
J C Lissitzky, and
M N Lavaut, and
F Kopp, and
N Pourreau-Schneider, and
J Jacquemier, and
M Toga
September 1986,
Chinese medical journal,
C Charpin, and
P M Martin, and
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M N Lavaut, and
F Kopp, and
N Pourreau-Schneider, and
J Jacquemier, and
M Toga
January 1985,
Acta neuropathologica,
C Charpin, and
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F Kopp, and
N Pourreau-Schneider, and
J Jacquemier, and
M Toga
December 1989,
The American Journal of dermatopathology,
C Charpin, and
P M Martin, and
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M N Lavaut, and
F Kopp, and
N Pourreau-Schneider, and
J Jacquemier, and
M Toga
January 1985,
Srpski arhiv za celokupno lekarstvo,
C Charpin, and
P M Martin, and
J C Lissitzky, and
M N Lavaut, and
F Kopp, and
N Pourreau-Schneider, and
J Jacquemier, and
M Toga
January 1989,
Zeitschrift fur Rechtsmedizin. Journal of legal medicine,
C Charpin, and
P M Martin, and
J C Lissitzky, and
M N Lavaut, and
F Kopp, and
N Pourreau-Schneider, and
J Jacquemier, and
M Toga
November 1992,
Biotechnic & histochemistry : official publication of the Biological Stain Commission,
C Charpin, and
P M Martin, and
J C Lissitzky, and
M N Lavaut, and
F Kopp, and
N Pourreau-Schneider, and
J Jacquemier, and
M Toga
February 1983,
Journal of immunological methods,
