Biomaterial Database

[Effect of the adenine level of a culture medium on the frequency of revertants in haploid yeasts]. 1985

V L Il'ina, and V I Korogodin, and C s Fajszi

The frequency of reversion from adenine auxotrophy in the yeast Saccharomyces cerevisiae increases with the decrease of adenine concentration in the medium: for the 8PG-59 strain (a ade2-192 rad2) the reversion frequency is 1.7 X 10(-8), 3.2 X 10(-6) and 1.8 X 10(-5) per cell division, the initial adenine concentrations being 10, 1 and 0.1 mg/l, respectively. An increase of the reversion frequency with the culture age in the stationary phase of growth is demonstrated using an improved method of registration of revertants, with the initial concentration of 0.1 mg/l of adenine in the medium. The reversion frequency was 9.1 X 10(-7) on the 7th day, 1.8 X 10(-6) on the 10th day and 1.8 X 10(-5) on the 14th day.

UI	MeSH Term	Description	Entries
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D000225	Adenine	A purine base and a fundamental unit of ADENINE NUCLEOTIDES.	Vitamin B 4,4, Vitamin B,B 4, Vitamin
D012441	Saccharomyces cerevisiae	A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.	Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker