-transparent.png){kind=logo}
A gene bank of Mycobacterium bovis BCG DNA in Escherichia coli was constructed by cloning Sau3A-cleaved mycobacterium DNA fragments into the lambda vector EMBL3. The expression of mycobacterial antigens was analyzed by Western blotting with hyperimmune rabbit sera. Among 770 clones tested, several were found that produced various mycobacterial antigens in low amounts, with concentrations generally close to the detection limit. One particular clone was chosen for further investigation. This clone produced a 64-kilodalton (kDa) antigen. By placing the lambda promoter PL in front of the structural gene of this antigen, an overproducing E. coli strain was obtained. Rocket-line immunoelectrophoresis experiments showed that antigens cross-reacting with the 64-kDa protein are present in a wide variety of mycobacteria and also in so-called purified protein derivatives which are routinely used for skin tests. Preliminary experiments indicate the presence of antibodies against the 64-kDa antigen in sera from tuberculosis patients.
J E Thole, and
H G Dauwerse, and
P K Das, and
D G Groothuis, and
L M Schouls, and
J D van Embden
October 1986,
Journal of bacteriology,
J E Thole, and
H G Dauwerse, and
P K Das, and
D G Groothuis, and
L M Schouls, and
J D van Embden
April 2005,
Wei sheng wu xue bao = Acta microbiologica Sinica,
J E Thole, and
H G Dauwerse, and
P K Das, and
D G Groothuis, and
L M Schouls, and
J D van Embden
January 2006,
Journal of biochemistry and molecular biology,
J E Thole, and
H G Dauwerse, and
P K Das, and
D G Groothuis, and
L M Schouls, and
J D van Embden
May 2006,
Protein expression and purification,
J E Thole, and
H G Dauwerse, and
P K Das, and
D G Groothuis, and
L M Schouls, and
J D van Embden
January 2010,
Biological research,
J E Thole, and
H G Dauwerse, and
P K Das, and
D G Groothuis, and
L M Schouls, and
J D van Embden
January 1996,
FEMS microbiology letters,
J E Thole, and
H G Dauwerse, and
P K Das, and
D G Groothuis, and
L M Schouls, and
J D van Embden
January 1994,
Veterinary microbiology,
J E Thole, and
H G Dauwerse, and
P K Das, and
D G Groothuis, and
L M Schouls, and
J D van Embden
August 2006,
Infection and immunity,
J E Thole, and
H G Dauwerse, and
P K Das, and
D G Groothuis, and
L M Schouls, and
J D van Embden
September 1988,
Journal of bacteriology,
J E Thole, and
H G Dauwerse, and
P K Das, and
D G Groothuis, and
L M Schouls, and
J D van Embden
November 1974,
Journal of the National Cancer Institute,
