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A yeast genomic DNA expression library in lambda gt11 antibody prepared against yeast DNA polymerase I were used to isolate the gene encoding DNA polymerase I. The identity of the DNA polymerase I gene was determined by several criteria. First, the clone-encoded protein is immunologically related to DNA polymerase I. Second, cells containing the gene cloned in the high copy number plasmid YEp24 overproduce the polymerase activity 4- to 5-fold as measured in yeast extracts. Finally, insertion of the gene downstream from a bacteriophage T7 promoter allows synthesis of yeast DNA polymerase I in Escherichia coli. Gene disruption and Southern hybridization experiments show that the polymerase is encoded by an essential, single copy gene. Examination of the germinated spores containing the disrupted gene reveals a defect in nuclear division and a terminal phenotype typical of replication mutants.
L M Johnson, and
M Snyder, and
L M Chang, and
R W Davis, and
J L Campbell
August 1986,
The Journal of biological chemistry,
L M Johnson, and
M Snyder, and
L M Chang, and
R W Davis, and
J L Campbell
May 1987,
Proceedings of the National Academy of Sciences of the United States of America,
L M Johnson, and
M Snyder, and
L M Chang, and
R W Davis, and
J L Campbell
December 1992,
The EMBO journal,
L M Johnson, and
M Snyder, and
L M Chang, and
R W Davis, and
J L Campbell
January 1985,
Current genetics,
L M Johnson, and
M Snyder, and
L M Chang, and
R W Davis, and
J L Campbell
June 1983,
Biochemistry,
L M Johnson, and
M Snyder, and
L M Chang, and
R W Davis, and
J L Campbell
July 1993,
Nucleic acids research,
L M Johnson, and
M Snyder, and
L M Chang, and
R W Davis, and
J L Campbell
September 1993,
Journal of bacteriology,
L M Johnson, and
M Snyder, and
L M Chang, and
R W Davis, and
J L Campbell
May 1991,
Cell,
L M Johnson, and
M Snyder, and
L M Chang, and
R W Davis, and
J L Campbell
October 1995,
Gene,
L M Johnson, and
M Snyder, and
L M Chang, and
R W Davis, and
J L Campbell
June 1984,
The Journal of biological chemistry,
