Biomaterial Database

Simultaneous assessment of red cell perfusion in skeletal muscle by laser Doppler flowmetry and video microscopy. 1985

K Tyml, and C G Ellis

The objective of this study was to compare temporal and spatial variations of the laser Doppler flowmeter output (V) with the corresponding variations of perfusion (cells/mm3 X mm/s) evaluated by video microscopy. The flowmetry and video microscopy sampled 2 mm3 (approx.) and 0.84 mm3 surface volumes of the sartorius muscle in anesthetized frogs, respectively. The overall ranges of the output and perfusion measurements were from 0.01 to 0.72 V and from 45 to 1404 cells/mm3 X mm/s. Within these ranges, temporal variations induced by muscle contraction correlated well (overall r = 0.91), but the spatial variations associated with the resting state correlated poorly (overall r = 0.45). When the penetration of the laser light was limited to 0.3-0.4 mm (to make the volumes sampled by both techniques more comparable) the overall r of the spatial comparison increased to 0.86. It is concluded that the flowmeter (1) is affected by red cell perfusion below the tissue depth of 0.3-0.4 mm, and (2) can follow both the temporal and spatial variations of red cell perfusion in the tissue examined.

UI	MeSH Term	Description	Entries
D007834	Lasers	An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.	Masers,Continuous Wave Lasers,Pulsed Lasers,Q-Switched Lasers,Continuous Wave Laser,Laser,Laser, Continuous Wave,Laser, Pulsed,Laser, Q-Switched,Lasers, Continuous Wave,Lasers, Pulsed,Lasers, Q-Switched,Maser,Pulsed Laser,Q Switched Lasers,Q-Switched Laser
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D008853	Microscopy	The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.	Compound Microscopy,Hand-Held Microscopy,Light Microscopy,Optical Microscopy,Simple Microscopy,Hand Held Microscopy,Microscopy, Compound,Microscopy, Hand-Held,Microscopy, Light,Microscopy, Optical,Microscopy, Simple
D009132	Muscles	Contractile tissue that produces movement in animals.	Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D010477	Perfusion	Treatment process involving the injection of fluid into an organ or tissue.	Perfusions
D012039	Regional Blood Flow	The flow of BLOOD through or around an organ or region of the body.	Blood Flow, Regional,Blood Flows, Regional,Flow, Regional Blood,Flows, Regional Blood,Regional Blood Flows
D002196	Capillaries	The minute vessels that connect arterioles and venules.	Capillary Beds,Sinusoidal Beds,Sinusoids,Bed, Sinusoidal,Beds, Sinusoidal,Capillary,Capillary Bed,Sinusoid,Sinusoidal Bed
D004301	Doppler Effect	Changes in the observed frequency of waves (as sound, light, or radio waves) due to the relative motion of source and observer. The effect was named for the 19th century Austrian physicist Johann Christian Doppler.	Doppler Shift,Effect, Doppler,Shift, Doppler
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man