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[Method of embedding cultures in situ in epoxy resins for comprehensive light and electron microscopic analysis]. 1979

V L Livshits

A simple method for making preparations for light and electron microscopy analysis both from tissue cultures and other biological objects is proposed. The techniques of in situ flat-parallel embedding in special forms between two layers of polyethylene phthalate (PEPh) film are described. The method makes the maximum use of all the properties of the PEPh film allowing to utilize it as a substrate-support for growing cultures and as a substrate for forming optical properties of the preparations. The preparations so made are suitable for preliminary examinations by light microscopy, photography, and marking the selected areas for subsequent flat and sagital ultratomy.

UI MeSH Term Description Entries
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D003584 Cytological Techniques Methods used to study CELLS. Cytologic Technics,Cytological Technic,Cytological Technics,Cytological Technique,Technic, Cytological,Technics, Cytological,Technique, Cytological,Techniques, Cytological,Cytologic Technic,Technic, Cytologic,Technics, Cytologic
D004853 Epoxy Resins Polymeric resins derived from OXIRANES and characterized by strength and thermosetting properties. Epoxy resins are often used as dental materials. Epoxy Resin,Resin, Epoxy,Resins, Epoxy

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