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A test system for the detection and quantitation of influenza virus hemagglutinin (HA) in biological specimens by solid-phase enzyme immunoassay (SPEIA) is described. The test system implies sequential adsorption on polystyrene base of anti-HA guinea pig gammaglobulins, detectable HA-containing antigen, monospecific anti-HA rabbit serum, and peroxidase antirabbit conjugate. Adsorption of the HA-containing antigen is run in the presence of a high concentration of nonionic detergent. The developed method is highly sensitive (0.3-0.5 ng in a specimen) and permits the detection and quantitation of HA in whole influenza virions, preparations of surface glycoproteins and in preparations of hemagglutinin recovered from virus particles. The analysis (after pre-sensitization of the polystyrene adsorbent) takes from 2 to 21/2 hours. The possibility of using the developed test system for control and standardization of inactivated influenza vaccines (whole-virion, split, and subunit) is discussed.
S Iu Veselov, and
M S Siniakov, and
I G Kharitonenkov, and
A K Gitel'man, and
A I Vasiaev
January 1988,
Trudy Instituta imeni Pastera,
S Iu Veselov, and
M S Siniakov, and
I G Kharitonenkov, and
A K Gitel'man, and
A I Vasiaev
January 1987,
Voprosy virusologii,
S Iu Veselov, and
M S Siniakov, and
I G Kharitonenkov, and
A K Gitel'man, and
A I Vasiaev
January 1984,
Voprosy virusologii,
S Iu Veselov, and
M S Siniakov, and
I G Kharitonenkov, and
A K Gitel'man, and
A I Vasiaev
January 1996,
Voprosy virusologii,
S Iu Veselov, and
M S Siniakov, and
I G Kharitonenkov, and
A K Gitel'man, and
A I Vasiaev
July 1985,
Zhurnal mikrobiologii, epidemiologii i immunobiologii,
S Iu Veselov, and
M S Siniakov, and
I G Kharitonenkov, and
A K Gitel'man, and
A I Vasiaev
January 1984,
Voprosy meditsinskoi khimii,
S Iu Veselov, and
M S Siniakov, and
I G Kharitonenkov, and
A K Gitel'man, and
A I Vasiaev
September 1987,
Zhurnal mikrobiologii, epidemiologii i immunobiologii,
S Iu Veselov, and
M S Siniakov, and
I G Kharitonenkov, and
A K Gitel'man, and
A I Vasiaev
January 1995,
Voprosy virusologii,
S Iu Veselov, and
M S Siniakov, and
I G Kharitonenkov, and
A K Gitel'man, and
A I Vasiaev
January 2002,
Virus research,
S Iu Veselov, and
M S Siniakov, and
I G Kharitonenkov, and
A K Gitel'man, and
A I Vasiaev
January 1988,
Laboratornoe delo,
