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To characterize further the regulatory mechanism modulating the expression of the Saccharomyces cerevisiae ARG3 gene, i.e., the specific repression by arginine and the general amino acid control, we analyzed by deletion the region upstream of that gene, determined the nucleotide sequence of operator-constitutive-like mutations affecting the specific regulation, and examined the behavior of an ARG3-galK fusion engineered at the initiating codon of ARG3. Similarly to what was observed in previous studies on the HIS3 and HIS4 genes, our data show that the general regulation acts as a positive control and that a sequence containing the nucleotide TGACTC, between positions -364 and -282 upstream of the transcription start, functions as a regulatory target site. This sequence contains the most proximal of the two TGACTC boxes identified in front of ARG3. While the general control appears to modulate transcription efficiency, the specific repression by arginine displays a posttranscriptional component (F. Messenguy and E. Dubois, Mol. Gen. Genet. 189:148-156, 1983). Our deletion and gene fusion analyses confirm that the specific and general controls operate independently of each other and assign the site responsible for arginine-specific repression to between positions -170 and +22. In keeping with this assignment, the two operator-constitutive-like mutations were localized at positions -80 and -46, respectively, and thus in a region which is not transcribed. We discuss a hypothesis accounting for the involvement of untranscribed DNA in a posttranscriptional control.
M Crabeel, and
R Huygen, and
K Verschueren, and
F Messenguy, and
K Tinel, and
R Cunin, and
N Glansdorff
February 1988,
Current genetics,
M Crabeel, and
R Huygen, and
K Verschueren, and
F Messenguy, and
K Tinel, and
R Cunin, and
N Glansdorff
September 1986,
Molecular and cellular biology,
M Crabeel, and
R Huygen, and
K Verschueren, and
F Messenguy, and
K Tinel, and
R Cunin, and
N Glansdorff
March 1990,
Molecular and cellular biology,
M Crabeel, and
R Huygen, and
K Verschueren, and
F Messenguy, and
K Tinel, and
R Cunin, and
N Glansdorff
September 1983,
Proceedings of the National Academy of Sciences of the United States of America,
M Crabeel, and
R Huygen, and
K Verschueren, and
F Messenguy, and
K Tinel, and
R Cunin, and
N Glansdorff
June 1988,
Microbiological reviews,
M Crabeel, and
R Huygen, and
K Verschueren, and
F Messenguy, and
K Tinel, and
R Cunin, and
N Glansdorff
December 1991,
Gene,
M Crabeel, and
R Huygen, and
K Verschueren, and
F Messenguy, and
K Tinel, and
R Cunin, and
N Glansdorff
February 1996,
The Journal of biological chemistry,
M Crabeel, and
R Huygen, and
K Verschueren, and
F Messenguy, and
K Tinel, and
R Cunin, and
N Glansdorff
January 1986,
CRC critical reviews in biochemistry,
M Crabeel, and
R Huygen, and
K Verschueren, and
F Messenguy, and
K Tinel, and
R Cunin, and
N Glansdorff
July 1981,
Molecular and cellular biology,
M Crabeel, and
R Huygen, and
K Verschueren, and
F Messenguy, and
K Tinel, and
R Cunin, and
N Glansdorff
September 1975,
European journal of biochemistry,
