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The modified method for isolation and purification of LT-enterotoxin from Escherichia coli strain H74-114 has been proposed. Modification is based on exclusion of concentration of bacterial cell lysates by 3-5 fold dilution and changing PH during chromatography. The proposed modification permitted to increase the yield of purified and active product 8-10 fold. The obtained preparations are homogenic due to PAAG-SDS data and immunochemical analysis. B-component of TL-enterotoxin is shown to consist of 4 subunits B in contrast to B-component of choleragen, consisting of 5 subunits. The mol mass of A component of both toxins is identical but in contrast to choleragen the component of LT-enterotoxin is not subject to proteolysis.
M V Stepanova, and
Iu V Vertiev, and
I A Shaginian, and
Iu V Ezepchuk
January 1983,
Padiatrie und Grenzgebiete,
M V Stepanova, and
Iu V Vertiev, and
I A Shaginian, and
Iu V Ezepchuk
January 1987,
Biulleten' eksperimental'noi biologii i meditsiny,
M V Stepanova, and
Iu V Vertiev, and
I A Shaginian, and
Iu V Ezepchuk
January 1986,
Medical microbiology and immunology,
M V Stepanova, and
Iu V Vertiev, and
I A Shaginian, and
Iu V Ezepchuk
January 1980,
Infection,
M V Stepanova, and
Iu V Vertiev, and
I A Shaginian, and
Iu V Ezepchuk
October 1987,
Biulleten' eksperimental'noi biologii i meditsiny,
M V Stepanova, and
Iu V Vertiev, and
I A Shaginian, and
Iu V Ezepchuk
January 1983,
Laboratornoe delo,
M V Stepanova, and
Iu V Vertiev, and
I A Shaginian, and
Iu V Ezepchuk
August 1984,
Veterinary microbiology,
M V Stepanova, and
Iu V Vertiev, and
I A Shaginian, and
Iu V Ezepchuk
January 1985,
Archivos de investigacion medica,
M V Stepanova, and
Iu V Vertiev, and
I A Shaginian, and
Iu V Ezepchuk
January 1986,
Medical microbiology and immunology,
M V Stepanova, and
Iu V Vertiev, and
I A Shaginian, and
Iu V Ezepchuk
January 1984,
Annales d'immunologie,
