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The preparation of test cells coated with specific IgG of known subclass is described. Such cells are required in the standardization of IgG subtyping reagents. At present, these test cells usually are prepared by coating cells with IgG myeloma paraproteins. However, these paraproteins may not be generally available and an alternative method is presented using more readily obtainable materials. Quantification of cell-bound IgG showed that the subclass did not affect the sensitivity of the antiglobulin test when using broad-spectrum anti-IgG and that the test cells produced had an optimal IgG coating. Reactions with subclass-specific antisera were however, considerably weaker that those obtained with broad-spectrum anti-IgG. A modified spin-layering technique for use with subclass-specific antisera is described.
V I Rawlinson, and
F Stratton, and
A H Merry
July 1976,
Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles,
V I Rawlinson, and
F Stratton, and
A H Merry
September 1968,
Clinical and experimental immunology,
V I Rawlinson, and
F Stratton, and
A H Merry
March 1985,
American journal of hematology,
V I Rawlinson, and
F Stratton, and
A H Merry
February 1970,
The Journal of general virology,
V I Rawlinson, and
F Stratton, and
A H Merry
January 1974,
Nouvelle revue francaise d'hematologie,
V I Rawlinson, and
F Stratton, and
A H Merry
January 1982,
Folia haematologica (Leipzig, Germany : 1928),
V I Rawlinson, and
F Stratton, and
A H Merry
March 1980,
Journal of clinical microbiology,
V I Rawlinson, and
F Stratton, and
A H Merry
April 1975,
Transplantation,
V I Rawlinson, and
F Stratton, and
A H Merry
January 1977,
Transfusion,
V I Rawlinson, and
F Stratton, and
A H Merry
January 1982,
Transfusion,
