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A precipitating anti-human mu chain monoclonal antibody (designated Tibi 82 McAb) was produced by the cell fusion technique. This McAb (isotype: IgG1 kappa) reacted by radioimmunoassay with all 10 human IgM proteins tested. In contrast, no reactivity was observed with IgG, IgA, IgE, lambda and kappa chains. 19 S IgM proteins were precipitated by Tibi 82 McAb using the Ouchterlony method under standard conditions. Hence specificity of this McAb for the C mu 2 domain was characterized by inhibition of precipitin reactions using human IgM fragments. Despite its narrow specificity for the C mu 2 domain, such a McAb could be used for IgM capture in the detection of specific IgM to Toxoplasma gondii employing the IgM immunosorbent agglutination assay (IgM-ISAGA). Tibi 82 McAb was compared with 3 anti-human IgM polyclonal reagents in the routine analysis of 117 sera. With 2 of them, a correlation coefficient of 0.976 was obtained and Tibi 82 McAb was more sensitive than the third polyclonal reagent tested. The IgM-ISAGA technique was shown to be reproducible using Tibi 82 McAb and similar anti-human mu chain McAbs could permit the wider development of reverse immunosorbent methods for the detection of specific IgM in various infectious diseases.
P Pouletty, and
J Kadouche, and
M Garcia-Gonzalez, and
E Mihaesco, and
G Desmonts, and
P Thulliez, and
H Thoannes, and
J M Pinon
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The Journal of infectious diseases,
P Pouletty, and
J Kadouche, and
M Garcia-Gonzalez, and
E Mihaesco, and
G Desmonts, and
P Thulliez, and
H Thoannes, and
J M Pinon
August 1987,
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P Pouletty, and
J Kadouche, and
M Garcia-Gonzalez, and
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G Desmonts, and
P Thulliez, and
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J M Pinon
September 1985,
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P Pouletty, and
J Kadouche, and
M Garcia-Gonzalez, and
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P Thulliez, and
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November 1986,
Journal of clinical microbiology,
P Pouletty, and
J Kadouche, and
M Garcia-Gonzalez, and
E Mihaesco, and
G Desmonts, and
P Thulliez, and
H Thoannes, and
J M Pinon
May 1984,
Nippon Ganka Gakkai zasshi,
P Pouletty, and
J Kadouche, and
M Garcia-Gonzalez, and
E Mihaesco, and
G Desmonts, and
P Thulliez, and
H Thoannes, and
J M Pinon
January 1986,
Revista do Instituto de Medicina Tropical de Sao Paulo,
P Pouletty, and
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M Garcia-Gonzalez, and
E Mihaesco, and
G Desmonts, and
P Thulliez, and
H Thoannes, and
J M Pinon
February 1980,
Infection and immunity,
P Pouletty, and
J Kadouche, and
M Garcia-Gonzalez, and
E Mihaesco, and
G Desmonts, and
P Thulliez, and
H Thoannes, and
J M Pinon
October 1985,
Journal of immunological methods,
P Pouletty, and
J Kadouche, and
M Garcia-Gonzalez, and
E Mihaesco, and
G Desmonts, and
P Thulliez, and
H Thoannes, and
J M Pinon
April 1986,
Presse medicale (Paris, France : 1983),
P Pouletty, and
J Kadouche, and
M Garcia-Gonzalez, and
E Mihaesco, and
G Desmonts, and
P Thulliez, and
H Thoannes, and
J M Pinon
December 1980,
Journal of clinical microbiology,
