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Insoluble extracts of human squamous cell carcinoma were solubilized by desoxycholate. The soluble extracts, after partial purification, were used to prepare antisera in guinea pigs. These antisera which were absorbed with insoluble extracts of lung, liver, kidney, spleen, carcinoembryonic antigen and non-specific cross-reacting antigen were commonly detected in all of the 7 squamous cell carcinomas. These antisera failed to reacted with adult organ extract, fetal organ homogenates or extracts from tumors of other histologic types by the methods of immuno-diffusion technique.
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