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Atherosclerotic lesions and xanthomas are characterized by the occurrence of cholesteryl ester (CE)-laden foam cells, which partly originate from macrophages. Little is known about the role of cyclo-oxygenase or lipoxygenase metabolites of arachidonic acid in the development of foam cells. In this study we investigated the influence of prostaglandins and inhibitors of the cyclo-oxygenase or the lipoxygenase pathway on CE accumulation in cultured human monocyte-derived macrophages. Accumulation of CE was achieved by incubation of the cells with acetylated low density lipoprotein (AcLDL). The stable prostacyclin analogue ZK 36 374 and prostaglandin E2 showed no effect on cellular CE storage. Similarly, the cyclo-oxygenase inhibitor indomethacin failed to influence AcLDL-induced CE accumulation. By contrast, however, the inhibitors of lipoxygenase activity nordihydroguaiaretic acid (NDGA) and BW 755 C markedly suppressed the accumulation of CE in monocyte-derived macrophages. The inhibitory effect of NDGA was dose-dependent. Incubation of the cells with the anti-oxidant vitamin E gave no significant reduction of CE accumulation. Our results indicate that inhibition of the lipoxygenase pathway of arachidonic acid metabolism in cultured monocyte-derived macrophages effectively decreases the rate of experimentally-induced CE accumulation.
J G van der Schroeff, and
L Havekes, and
A M Weerheim, and
J J Emeis, and
B J Vermeer
March 1993,
Atherosclerosis,
J G van der Schroeff, and
L Havekes, and
A M Weerheim, and
J J Emeis, and
B J Vermeer
October 1985,
Biochimica et biophysica acta,
J G van der Schroeff, and
L Havekes, and
A M Weerheim, and
J J Emeis, and
B J Vermeer
February 1982,
Proceedings of the National Academy of Sciences of the United States of America,
J G van der Schroeff, and
L Havekes, and
A M Weerheim, and
J J Emeis, and
B J Vermeer
April 1991,
Clinical immunology and immunopathology,
J G van der Schroeff, and
L Havekes, and
A M Weerheim, and
J J Emeis, and
B J Vermeer
January 1991,
Arteriosclerosis and thrombosis : a journal of vascular biology,
J G van der Schroeff, and
L Havekes, and
A M Weerheim, and
J J Emeis, and
B J Vermeer
May 1988,
Diabetes,
J G van der Schroeff, and
L Havekes, and
A M Weerheim, and
J J Emeis, and
B J Vermeer
April 1980,
Proceedings of the National Academy of Sciences of the United States of America,
J G van der Schroeff, and
L Havekes, and
A M Weerheim, and
J J Emeis, and
B J Vermeer
October 1982,
Lipids,
J G van der Schroeff, and
L Havekes, and
A M Weerheim, and
J J Emeis, and
B J Vermeer
May 1987,
Atherosclerosis,
J G van der Schroeff, and
L Havekes, and
A M Weerheim, and
J J Emeis, and
B J Vermeer
July 2000,
Journal of cardiovascular pharmacology,
