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Sterol carrier protein 2 (SCP-2) participates in the microsomal conversion of lanosterol to cholesterol, in the conversion of cholesterol to cholesterol ester, and in intracellular cholesterol transfers. The stoichiometry of binding between cholesterol and SCP-2 is 1:1. However, reports have appeared attributing sterol carrier protein activity to a protein preparation identical to hepatic fatty acid-binding protein (FABP). Therefore, the present investigation was conducted to compare homogeneous preparations of FABP and SCP-2 with respect to their capacities to participate as carrier proteins in reactions involving sterols or fatty acids. The results show that SCP-2 and FABP have separate and distinct physiological functions, with SCP-2 participating in reactions involving sterols and FABP participating in reactions involving fatty acid binding and/or transport. Furthermore, there is no overlap in substrate specificities, i.e. FABP does not possess sterol carrier protein activity and SCP-2 does not specifically bind or transport fatty acid. As long as only small quantities of organic solvent (1.6 volume %) were used for substrate addition, the sterol delta 7-reductase liver microsomal assay for SCP-2 correlated well with the physiologically relevant assays employed in the reconstituted adrenal system. The sterol carrier protein activity previously attributed to rat hepatic FABP is explained by the presence of significant quantities of propylene glycol (15 volume %) or Tween 80 in the assay procedure.
T J Scallen, and
B J Noland, and
K L Gavey, and
N M Bass, and
R K Ockner, and
R Chanderbhan, and
G V Vahouny
September 1996,
Journal of lipid research,
T J Scallen, and
B J Noland, and
K L Gavey, and
N M Bass, and
R K Ockner, and
R Chanderbhan, and
G V Vahouny
October 2019,
Lipids,
T J Scallen, and
B J Noland, and
K L Gavey, and
N M Bass, and
R K Ockner, and
R Chanderbhan, and
G V Vahouny
December 1996,
The Journal of biological chemistry,
T J Scallen, and
B J Noland, and
K L Gavey, and
N M Bass, and
R K Ockner, and
R Chanderbhan, and
G V Vahouny
September 2006,
Archives of biochemistry and biophysics,
T J Scallen, and
B J Noland, and
K L Gavey, and
N M Bass, and
R K Ockner, and
R Chanderbhan, and
G V Vahouny
September 2003,
Journal of lipid research,
T J Scallen, and
B J Noland, and
K L Gavey, and
N M Bass, and
R K Ockner, and
R Chanderbhan, and
G V Vahouny
December 1999,
The Journal of biological chemistry,
T J Scallen, and
B J Noland, and
K L Gavey, and
N M Bass, and
R K Ockner, and
R Chanderbhan, and
G V Vahouny
August 2014,
Molecular neurobiology,
T J Scallen, and
B J Noland, and
K L Gavey, and
N M Bass, and
R K Ockner, and
R Chanderbhan, and
G V Vahouny
February 1997,
Biochemistry,
T J Scallen, and
B J Noland, and
K L Gavey, and
N M Bass, and
R K Ockner, and
R Chanderbhan, and
G V Vahouny
March 2003,
Biochemistry,
T J Scallen, and
B J Noland, and
K L Gavey, and
N M Bass, and
R K Ockner, and
R Chanderbhan, and
G V Vahouny
July 2009,
Biophysical journal,
