-transparent.png){kind=logo}
A method for isolating ferritin from human term placenta was described. The placenta was homogenized in water containing protease inhibitor and heated at 70 degrees C. The ferritin was precipitated with ammonium sulfate at pH 5.2 and purified by repeated cycles of ultracentrifugation and molecular sieve chromatography through Sepharose 4B columns. Isoelectric focusing revealed a broad spectrum of isoferritins. These isoferritins were separated by ion-exchange chromatography on Sephadex A-25 at pH 7.5 and stepwise elution with increasing concentrations of NaCl. By this method "basic," "intermediate," and "acid" isoferritins were separated. The most basic placental isoferritin was shown to be identical to splenic ferritin by isoelectric focusing, subunit analysis, and fluorescent ELISA. The acid placental isoferritin had similar characteristics to heart-type ferritin. It was suggested that the easily available placental tissue could serve as a source for human isoferritins in research and in clinical assays.
A M Konijn, and
R Tal, and
R Levy, and
Y Matzner
June 1987,
Biotechnology and bioengineering,
A M Konijn, and
R Tal, and
R Levy, and
Y Matzner
December 1969,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
A M Konijn, and
R Tal, and
R Levy, and
Y Matzner
December 1974,
Cancer research,
A M Konijn, and
R Tal, and
R Levy, and
Y Matzner
January 2005,
Stem cells (Dayton, Ohio),
A M Konijn, and
R Tal, and
R Levy, and
Y Matzner
August 1984,
Archives of biochemistry and biophysics,
A M Konijn, and
R Tal, and
R Levy, and
Y Matzner
December 1984,
Clinica chimica acta; international journal of clinical chemistry,
A M Konijn, and
R Tal, and
R Levy, and
Y Matzner
January 2024,
Methods in molecular biology (Clifton, N.J.),
A M Konijn, and
R Tal, and
R Levy, and
Y Matzner
October 2021,
Bio-protocol,
A M Konijn, and
R Tal, and
R Levy, and
Y Matzner
January 1977,
Blood,
A M Konijn, and
R Tal, and
R Levy, and
Y Matzner
January 1979,
Progress in hematology,
