-transparent.png){kind=logo}
The purified bacteriocin was sensitive to proteolytic enzymes such as trypsin, alpha-chymotrypsin and pronase, but resistant to papain and pepsin. Lowering the pH of the bacteriocin caused precipitation, and the Abs280 of the supernatant reached a minimum at pH 3.6, suggesting that this is the isoelectric point. Such a pH value coincides with minimum bacteriocin activity. The effect of the bacteriocin was bactericidal rather than bacteriolytic and concentration dependent. Treatment of Streptococcus faecalis ODU with Rm-10 bacteriocin led rapidly to the cessation of biosynthesis of macromolecules, DNA, RNA and protein. Electron microscopy showed bacteriocin fibres attached in aggregated bundles to target cells; after ultra-sonication or detergent treatment they were individually attached to the cell surface and, at the same time, the apparent bacteriocin activity had doubled. Bacteriocin activity was not altered by filter-sterilized saliva components. Oral rinsing with Rm-10 bacteriocin reduced the number of viable salivary bacteria after 20 min, suggesting a possible therapeutic use for Rm-10 bacteriocin.
H Fukushima, and
J Kelstrup, and
S Fukushima, and
T Umemoto, and
A Kaibori, and
H Sagawa
January 1982,
Archives of oral biology,
H Fukushima, and
J Kelstrup, and
S Fukushima, and
T Umemoto, and
A Kaibori, and
H Sagawa
October 1990,
Inflammation,
H Fukushima, and
J Kelstrup, and
S Fukushima, and
T Umemoto, and
A Kaibori, and
H Sagawa
January 1985,
Archives of oral biology,
H Fukushima, and
J Kelstrup, and
S Fukushima, and
T Umemoto, and
A Kaibori, and
H Sagawa
January 1980,
Archives of oral biology,
H Fukushima, and
J Kelstrup, and
S Fukushima, and
T Umemoto, and
A Kaibori, and
H Sagawa
May 1984,
Infection and immunity,
H Fukushima, and
J Kelstrup, and
S Fukushima, and
T Umemoto, and
A Kaibori, and
H Sagawa
May 1978,
Infection and immunity,
H Fukushima, and
J Kelstrup, and
S Fukushima, and
T Umemoto, and
A Kaibori, and
H Sagawa
May 1986,
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology,
H Fukushima, and
J Kelstrup, and
S Fukushima, and
T Umemoto, and
A Kaibori, and
H Sagawa
January 1992,
Microbiology and immunology,
H Fukushima, and
J Kelstrup, and
S Fukushima, and
T Umemoto, and
A Kaibori, and
H Sagawa
December 1975,
Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles,
H Fukushima, and
J Kelstrup, and
S Fukushima, and
T Umemoto, and
A Kaibori, and
H Sagawa
June 1985,
The Journal of biological chemistry,
