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We have developed a kinetic immunonephelometric method for the determination of retinol-binding protein and modified the method of Jacob et al (Clin Chem 1983; 29: 564) for the determination of transthyretin (prealbumin) in neonatal serum specimens from small, premature infants. The methodologies allow detection of 17.5 mg/l transthyretin and 1.7 mg/l retinol-binding protein in 25 microliter of serum. Between-run precision studies using pooled neonatal serum gave CV values of 3% and 5-6% for transthyretin and retinol-binding protein, respectively. Results obtained for neonatal specimens using this method agreed well with those obtained for the same specimens using radial immunodiffusion. Mean (SD) serum concentrations for 39 neonatal specimens were 100.4 (46.6) and 26.3 (10.8) mg/l for transthyretin and retinol-binding protein, respectively.
F Gulamali, and
T E Keegan, and
B Numerof, and
B Chrenka, and
F Wirth, and
P A Pleban
January 2010,
Journal of renal nutrition : the official journal of the Council on Renal Nutrition of the National Kidney Foundation,
F Gulamali, and
T E Keegan, and
B Numerof, and
B Chrenka, and
F Wirth, and
P A Pleban
November 1984,
Annals of clinical biochemistry,
F Gulamali, and
T E Keegan, and
B Numerof, and
B Chrenka, and
F Wirth, and
P A Pleban
October 2000,
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F Gulamali, and
T E Keegan, and
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B Chrenka, and
F Wirth, and
P A Pleban
December 2010,
ACS chemical biology,
F Gulamali, and
T E Keegan, and
B Numerof, and
B Chrenka, and
F Wirth, and
P A Pleban
January 2013,
PloS one,
F Gulamali, and
T E Keegan, and
B Numerof, and
B Chrenka, and
F Wirth, and
P A Pleban
May 1983,
Clinical chemistry,
F Gulamali, and
T E Keegan, and
B Numerof, and
B Chrenka, and
F Wirth, and
P A Pleban
November 1992,
Biochemistry,
F Gulamali, and
T E Keegan, and
B Numerof, and
B Chrenka, and
F Wirth, and
P A Pleban
January 2004,
Vitamins and hormones,
F Gulamali, and
T E Keegan, and
B Numerof, and
B Chrenka, and
F Wirth, and
P A Pleban
November 1994,
Journal of molecular biology,
F Gulamali, and
T E Keegan, and
B Numerof, and
B Chrenka, and
F Wirth, and
P A Pleban
July 1998,
Developmental dynamics : an official publication of the American Association of Anatomists,
