Biomaterial Database

Characteristics of antiviral and anticellular activities of human recombinant interferon-gamma. 1985

K Gomi, and M Morimoto, and N Nakamizo

The antiviral and anticellular activities of recombinant human interferon-gamma (Re-IFN-gamma) were compared with those of natural human interferon-gamma (IFN-gamma), natural human interferon-alpha (IFN-alpha), and recombinant human interferon-beta (ReIFN-beta). ReIFN-gamma and IFN-gamma induced the antiviral state in FL cells against Sindbis virus more rapidly than IFN-alpha or ReIFN-beta. The antiviral state induced by a short exposure time to IFN-gamma declined rapidly after the removal of IFN-gamma and the incubation of FL cells in fresh medium, whereas that induced by ReIFN-gamma was persistent. However, other characteristics of the antiviral activity of ReIFN-gamma were shown to be very similar to those of IFN-gamma (e.g., stability at 56 degrees or pH 2.0, and neutralization by anti-IFN serum). The anticellular spectrum of ReIFN-gamma or IFN-gamma against 26 human cell lines differed in some respects from that of IFN-alpha or ReIFN-beta, since ReIFN-gamma and IFN-gamma were more effective at inhibiting the growth of human carcinoma or sarcoma cell lines. The IC50 (IFN concentration required for 50% growth inhibition) values of ReIFN-gamma were several times smaller than those of IFN-gamma in all cell lines sensitive to both IFNs. ReIFN-gamma and IFN-gamma, but not IFN-alpha or ReIFN-beta, were cytotoxic against HeLa S3 cells. These cytotoxicities of both IFNs were inactivated by treatment at 56 degrees or pH 2.0 and by anti-ReIFN-gamma serum. These results indicate that the characteristics of antiviral and anticellular activities of ReIFN-gamma are somewhat different from those of other IFNs including IFN-gamma.

UI	MeSH Term	Description	Entries
D007371	Interferon-gamma	The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.	Interferon Type II,Interferon, Immune,gamma-Interferon,Interferon, gamma,Type II Interferon,Immune Interferon,Interferon, Type II
D008175	Lung Neoplasms	Tumors or cancer of the LUNG.	Cancer of Lung,Lung Cancer,Pulmonary Cancer,Pulmonary Neoplasms,Cancer of the Lung,Neoplasms, Lung,Neoplasms, Pulmonary,Cancer, Lung,Cancer, Pulmonary,Cancers, Lung,Cancers, Pulmonary,Lung Cancers,Lung Neoplasm,Neoplasm, Lung,Neoplasm, Pulmonary,Pulmonary Cancers,Pulmonary Neoplasm
D008223	Lymphoma	A general term for various neoplastic diseases of the lymphoid tissue.	Germinoblastoma,Lymphoma, Malignant,Reticulolymphosarcoma,Sarcoma, Germinoblastic,Germinoblastic Sarcoma,Germinoblastic Sarcomas,Germinoblastomas,Lymphomas,Lymphomas, Malignant,Malignant Lymphoma,Malignant Lymphomas,Reticulolymphosarcomas,Sarcomas, Germinoblastic
D008545	Melanoma	A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)	Malignant Melanoma,Malignant Melanomas,Melanoma, Malignant,Melanomas,Melanomas, Malignant
D009374	Neoplasms, Experimental	Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.	Experimental Neoplasms,Experimental Neoplasm,Neoplasm, Experimental
D002455	Cell Division	The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.	M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D006358	Hot Temperature	Presence of warmth or heat or a temperature notably higher than an accustomed norm.	Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D006367	HeLa Cells	The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays.	Cell, HeLa,Cells, HeLa,HeLa Cell