Biomaterial Database

[Combined effect of heat shock and ecdysone on the transcription of Drosophila melanogaster polytene chromosomes]. 1985

M B Evgen'ev, and O G Zatsepina, and V T Kakpakov, and I E Vlasova

The combined action of ecdysone and temperature elevation (heat shock) on D. melanogaster polytene chromosome transcription has been investigated. It was shown that heat shock while blocking the transcription in ecdysone--induced puffs is not effective in decreasing their size. Thus we are able to observe chromatin decondensation without transcription. The indirect immunofluorescence using an antiserum directed against DNA/RNA hybrids revealed an intense fluorescence in the polytene chromosomes of heat shocked larvae both in the actively transcribed heat shock puffs and in ecdysone stimulated "primary puffs" with blocked transcription. Ecdysone introduced into the media during heat shock is unable to induce any puffs. On the other hand the hormone introduced into the media after temperature elevation (30' of heat shock + 1 hour of ecdysone stimulation) induces some of the "primary puffs". A new approach for cloning the genes inducible by ecdysone and other inducible loci as well has been developed using the data obtained.

UI	MeSH Term	Description	Entries
D002875	Chromosomes	In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)	Chromosome
D004331	Drosophila melanogaster	A species of fruit fly frequently used in genetics because of the large size of its chromosomes.	D. melanogaster,Drosophila melanogasters,melanogaster, Drosophila
D004440	Ecdysone	A steroid hormone that regulates the processes of MOLTING or ecdysis in insects.	Molting Hormone
D006358	Hot Temperature	Presence of warmth or heat or a temperature notably higher than an accustomed norm.	Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012469	Salivary Glands	Glands that secrete SALIVA in the MOUTH. There are three pairs of salivary glands (PAROTID GLAND; SUBLINGUAL GLAND; SUBMANDIBULAR GLAND).	Gland, Salivary,Glands, Salivary,Salivary Gland
D014158	Transcription, Genetic	The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.	Genetic Transcription
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In