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To date no nucleic acid has been found in the purified infectious agent which causes the spongiform encephalopathy known as scrapie. In an attempt to identify a unique scrapie virus-associated messenger RNA in tissues of infected animals, we have synthesized an oligonucleotide probe complementary to the mRNA sequence corresponding to the amino-acid sequence of the prion protein, PrP27-30 (ref. 1). We report here that, with this probe, a complementary DNA clone representing PrP27-30 was obtained from scrapie-infected mouse brain; the DNA sequence of this clone could be translated into a protein that matches exactly the published sequence of PrP27-30. The cDNA clone hybridized to a single 2.4-2.5-kilobase (kb) mRNA from both normal and scrapie-infected brain. Thus, the PrP27-30 mRNA is not uniquely associated with scrapie infectivity, suggesting that PrP27-30 may be a normal component of mouse and hamster brain.
B Chesebro, and
R Race, and
K Wehrly, and
J Nishio, and
M Bloom, and
D Lechner, and
S Bergstrom, and
K Robbins, and
L Mayer, and
J M Keith
May 1985,
Cell,
B Chesebro, and
R Race, and
K Wehrly, and
J Nishio, and
M Bloom, and
D Lechner, and
S Bergstrom, and
K Robbins, and
L Mayer, and
J M Keith
March 1993,
Cell biochemistry and function,
B Chesebro, and
R Race, and
K Wehrly, and
J Nishio, and
M Bloom, and
D Lechner, and
S Bergstrom, and
K Robbins, and
L Mayer, and
J M Keith
February 1989,
Journal of comparative pathology,
B Chesebro, and
R Race, and
K Wehrly, and
J Nishio, and
M Bloom, and
D Lechner, and
S Bergstrom, and
K Robbins, and
L Mayer, and
J M Keith
September 2006,
The Veterinary record,
B Chesebro, and
R Race, and
K Wehrly, and
J Nishio, and
M Bloom, and
D Lechner, and
S Bergstrom, and
K Robbins, and
L Mayer, and
J M Keith
January 2006,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
B Chesebro, and
R Race, and
K Wehrly, and
J Nishio, and
M Bloom, and
D Lechner, and
S Bergstrom, and
K Robbins, and
L Mayer, and
J M Keith
November 1992,
Neuroscience letters,
B Chesebro, and
R Race, and
K Wehrly, and
J Nishio, and
M Bloom, and
D Lechner, and
S Bergstrom, and
K Robbins, and
L Mayer, and
J M Keith
June 1994,
Annals of the New York Academy of Sciences,
B Chesebro, and
R Race, and
K Wehrly, and
J Nishio, and
M Bloom, and
D Lechner, and
S Bergstrom, and
K Robbins, and
L Mayer, and
J M Keith
July 2001,
Proceedings of the National Academy of Sciences of the United States of America,
B Chesebro, and
R Race, and
K Wehrly, and
J Nishio, and
M Bloom, and
D Lechner, and
S Bergstrom, and
K Robbins, and
L Mayer, and
J M Keith
July 1992,
The Journal of general virology,
B Chesebro, and
R Race, and
K Wehrly, and
J Nishio, and
M Bloom, and
D Lechner, and
S Bergstrom, and
K Robbins, and
L Mayer, and
J M Keith
January 2004,
Journal of neuropathology and experimental neurology,
