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Successful application of the diffusion-in-gel-enzyme-linked immunosorbent assay (DIG-ELISA) in onchocerciasis. 1985

A Gómez-Priego, and J F Paniagua-Solís, and R A Rivas-Alcalá, and M T Ruenes-Meza

The recently described DIG-ELISA test was evaluated on 38 sera from patients with onchocerciasis, using a soluble, crude extract of Onchocerca volvulus dissected out from glycerol-preserved nodules. Results showed that the diameters of the orange-yellow antibody-antigen reaction zones obtained with infected sera were significantly greater than those obtained with normal human sera. A close correlation was observed between the diameters of the reaction zones and antibody concentration (expressed as log of the serum dilution), indicating the potential utility of this test in the quantitative determination of serum antibodies. Because the test is easily performed, it can be used under field conditions when an immediate quantitative determination of serum antibodies is needed.

UI MeSH Term Description Entries
D009855 Onchocerciasis Infection with nematodes of the genus ONCHOCERCA. Characteristics include the presence of firm subcutaneous nodules filled with adult worms, PRURITUS, and ocular lesions. Onchocerciases
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005779 Immunodiffusion Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction. Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000906 Antibodies Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).

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