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Anti-murine interleukin 2 (IL-2) receptor monoclonal antibodies (mAb) were made from rats immunized with murine cytotoxic lymphocytes. One mAb, designated M7/20, strongly inhibited the proliferation of both IL-2 dependent CTLL-2 cells and concanavalin A (Con A)-induced T-cell blasts. Inhibition was linearly dependent on the concentrations of both M7/20 and IL-2. Utilizing FACS analysis, M7/20 was shown to bind selectively to mitogen-activated T lymphocytes and, to a lesser degree, to activated B lymphocytes. 125I-Labeled M7/20 binding assays indicated that 48-hr Con A-induced T-cell blasts possessed 89,000 binding sites/cell with a Kd of 1.2 X 10(-9) M. Competitive binding analyses indicated that M7/20 and IL-2 occupy the same or overlapping cell surface sites. Preliminary biochemical characterization of M7/20 immunoprecipitates of detergent extracts from both surface-iodinated and internally D-[3H]glucosamine-labeled T lymphoblasts indicated that the murine IL-2 receptor is an N-glycosylated 58,000-Da glycoprotein. Together these results suggest that mAb M7/20 binds at or near the IL-2-binding epitope on the murine IL-2 receptor and, thus, upon manipulation may act as an IL-2 agonist.
G N Gaulton, and
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S Maddock, and
T Springer, and
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T B Strom
May 1984,
Journal of immunology (Baltimore, Md. : 1950),
G N Gaulton, and
J Bangs, and
S Maddock, and
T Springer, and
D D Eardley, and
T B Strom
September 1983,
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G N Gaulton, and
J Bangs, and
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T Springer, and
D D Eardley, and
T B Strom
June 1986,
Journal of immunology (Baltimore, Md. : 1950),
G N Gaulton, and
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G N Gaulton, and
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G N Gaulton, and
J Bangs, and
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T Springer, and
D D Eardley, and
T B Strom
December 2002,
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G N Gaulton, and
J Bangs, and
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November 1989,
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G N Gaulton, and
J Bangs, and
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T B Strom
February 1987,
Transplantation proceedings,
G N Gaulton, and
J Bangs, and
S Maddock, and
T Springer, and
D D Eardley, and
T B Strom
February 1987,
Transplantation proceedings,
G N Gaulton, and
J Bangs, and
S Maddock, and
T Springer, and
D D Eardley, and
T B Strom
December 1990,
Human immunology,
