BIOMAT Database Logo BIOMAT Database Logo

Persistence of DNA lesions and the cytological cancellation of sister chromatid exchanges. 1985

J B Schvartzman, and V J Goyanes, and A Campos, and A M Lage, and C Veiras, and M C Silva, and S Ramos

The ability of UV light, mitomycin C and ionizing radiation to induce the formation of sister chromatid exchanges (SCEs) at the same locus in successive cell generations was investigated in human lymphocytes. Cells were exposed to the DNA damaging agents after they had completed their first round of DNA replication, and SCEs were examined at the third division in chromosomes that had been differentially stained three ways. Although some of these treatments induced long-lived lesions that increased the frequency of SCEs in successive cell generations, none of the lesions led to the formation of consecutive SCEs at the same locus in successive cell generations. This observation seriously challenges the hypothesis that SCE cancellation results as a consequence of persistence of the lesions induced by these agents.

UI MeSH Term Description Entries
D008214 Lymphocytes White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS. Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D008937 Mitomycins A group of methylazirinopyrroloindolediones obtained from certain Streptomyces strains. They are very toxic antibiotics used as ANTINEOPLASTIC AGENTS in some solid tumors. PORFIROMYCIN and MITOMYCIN are the most useful members of the group.
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002877 Chromosomes, Human Very long DNA molecules and associated proteins, HISTONES, and non-histone chromosomal proteins (CHROMOSOMAL PROTEINS, NON-HISTONE). Normally 46 chromosomes, including two sex chromosomes are found in the nucleus of human cells. They carry the hereditary information of the individual. Chromosome, Human,Human Chromosome,Human Chromosomes
D003078 Colchicine A major alkaloid from Colchicum autumnale L. and found also in other Colchicum species. Its primary therapeutic use is in the treatment of gout, but it has been used also in the therapy of familial Mediterranean fever (PERIODIC DISEASE). Colchicine, (+-)-Isomer,Colchicine, (R)-Isomer
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000903 Antibiotics, Antineoplastic Chemical substances, produced by microorganisms, inhibiting or preventing the proliferation of neoplasms. Antineoplastic Antibiotics,Cytotoxic Antibiotics,Antibiotics, Cytotoxic
D012854 Sister Chromatid Exchange An exchange of segments between the sister chromatids of a chromosome, either between the sister chromatids of a meiotic tetrad or between the sister chromatids of a duplicated somatic chromosome. Its frequency is increased by ultraviolet and ionizing radiation and other mutagenic agents and is particularly high in BLOOM SYNDROME. Chromatid Exchange, Sister,Chromatid Exchanges, Sister,Exchange, Sister Chromatid,Exchanges, Sister Chromatid,Sister Chromatid Exchanges
D014466 Ultraviolet Rays That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants. Actinic Rays,Black Light, Ultraviolet,UV Light,UV Radiation,Ultra-Violet Rays,Ultraviolet Light,Ultraviolet Radiation,Actinic Ray,Light, UV,Light, Ultraviolet,Radiation, UV,Radiation, Ultraviolet,Ray, Actinic,Ray, Ultra-Violet,Ray, Ultraviolet,Ultra Violet Rays,Ultra-Violet Ray,Ultraviolet Black Light,Ultraviolet Black Lights,Ultraviolet Radiations,Ultraviolet Ray

Related Publications

J B Schvartzman, and V J Goyanes, and A Campos, and A M Lage, and C Veiras, and M C Silva, and S Ramos
Fate of DNA lesions that elicit sister-chromatid exchanges.
September 1990, Mutation research,
J B Schvartzman, and V J Goyanes, and A Campos, and A M Lage, and C Veiras, and M C Silva, and S Ramos
DNA repair and sister chromatid exchanges.
January 1984, Basic life sciences,
J B Schvartzman, and V J Goyanes, and A Campos, and A M Lage, and C Veiras, and M C Silva, and S Ramos
Recombinational DNA repair and sister chromatid exchanges.
August 1974, Mutation research,
J B Schvartzman, and V J Goyanes, and A Campos, and A M Lage, and C Veiras, and M C Silva, and S Ramos
Sister chromatid exchanges.
January 1980, Advances in human genetics,
J B Schvartzman, and V J Goyanes, and A Campos, and A M Lage, and C Veiras, and M C Silva, and S Ramos
Sister-chromatid exchanges.
May 1979, Genetics,
J B Schvartzman, and V J Goyanes, and A Campos, and A M Lage, and C Veiras, and M C Silva, and S Ramos
Sister chromatid exchanges and heterochromatin.
January 1981, Human genetics,
J B Schvartzman, and V J Goyanes, and A Campos, and A M Lage, and C Veiras, and M C Silva, and S Ramos
Analysis of sister-chromatid exchanges.
May 2001, Current protocols in human genetics,
J B Schvartzman, and V J Goyanes, and A Campos, and A M Lage, and C Veiras, and M C Silva, and S Ramos
Sister chromatid exchanges in isolabeling.
February 1974, Experimental cell research,
J B Schvartzman, and V J Goyanes, and A Campos, and A M Lage, and C Veiras, and M C Silva, and S Ramos
[Chromatid sister exchanges in neonates].
January 1985, Przeglad lekarski,
J B Schvartzman, and V J Goyanes, and A Campos, and A M Lage, and C Veiras, and M C Silva, and S Ramos
Sister chromatid exchanges in photochemotherapy.
October 1977, The Journal of investigative dermatology,
Copied contents to your clipboard!