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Recombinant human interferon-gamma (rHuIFN-gamma) and natural human tumor necrosis factor beta (nHuTNF-beta) (previously called lymphotoxin), purified to homogeneity, were used to assess their effects on certain functions of human polymorphonuclear neutrophils (PMN) in vitro. The treatment of PMN with 100 U of either rHuIFN-gamma or nHuTNF-beta for 20 min significantly increased their ability to phagocytize 1.5-microns latex beads as detected by flow cytometry. Preparations of recombinant human TNF-beta (rHuTNF-beta) showed activities similar to those of its natural counterpart in activating phagocytosis. In addition, a significant enhancement in PMN-mediated antibody-dependent cellular cytotoxicity was observed after treatment for 2 hr with IFN gamma and both TNF-alpha and TNF-beta. The enhancement by treatment with a combination of rHuIFN-gamma and nHuTNF-beta exceeded the enhancement caused by either agent alone. We also show that although lipopolysaccharide (LPS) is a potent stimulator of PMN function, polymyxin B can block LPS-induced but not lymphokine-induced activation. These data demonstrate new activities for both TNF-alpha and TNF-beta in augmenting the phagocytic and cytotoxic activities of PMN.
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B B Aggarwal, and
E Rinderknecht, and
L P Svedersky, and
B S Finkle, and
M A Palladino
March 1986,
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M R Shalaby, and
B B Aggarwal, and
E Rinderknecht, and
L P Svedersky, and
B S Finkle, and
M A Palladino
May 2004,
Immunology,
M R Shalaby, and
B B Aggarwal, and
E Rinderknecht, and
L P Svedersky, and
B S Finkle, and
M A Palladino
November 1986,
Journal of immunology (Baltimore, Md. : 1950),
M R Shalaby, and
B B Aggarwal, and
E Rinderknecht, and
L P Svedersky, and
B S Finkle, and
M A Palladino
December 1983,
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M R Shalaby, and
B B Aggarwal, and
E Rinderknecht, and
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M A Palladino
August 1990,
Infection and immunity,
M R Shalaby, and
B B Aggarwal, and
E Rinderknecht, and
L P Svedersky, and
B S Finkle, and
M A Palladino
January 1992,
Immunology series,
M R Shalaby, and
B B Aggarwal, and
E Rinderknecht, and
L P Svedersky, and
B S Finkle, and
M A Palladino
June 1989,
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M R Shalaby, and
B B Aggarwal, and
E Rinderknecht, and
L P Svedersky, and
B S Finkle, and
M A Palladino
October 1991,
European journal of immunology,
M R Shalaby, and
B B Aggarwal, and
E Rinderknecht, and
L P Svedersky, and
B S Finkle, and
M A Palladino
April 1989,
The Journal of surgical research,
M R Shalaby, and
B B Aggarwal, and
E Rinderknecht, and
L P Svedersky, and
B S Finkle, and
M A Palladino
October 1990,
Immunology letters,
