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We examined effects of small dose (1 microM or less) of exogenous 12-hydroperoxy-5,8,10,14-eicosatetraenoic acid (12-HPETE) on the formation of cyclooxygenase products from exogenous arachidonic acid (AA) in washed human platelets. With a simultaneous addition of AA, 12-HPETE did not affect the formation of thromboxane (TX)B2 and 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT). However, by being preincubated with platelets before an addition of AA, 0.1 microM or greater of 12-HPETE inhibited the formation of TXB2 and HHT dose-dependently. In addition, the inhibitory effect of 12-HPETE increased as the preincubation time was prolonged. These results suggest that 12-HPETE is a strong inhibitor for the cyclooxygenase pathway.
Y Hashimoto, and
C Naito, and
T Teramoto, and
H Kato, and
M Kinoshita, and
M Kawamura, and
H Hayashi, and
H Oka
August 1979,
Proceedings of the National Academy of Sciences of the United States of America,
Y Hashimoto, and
C Naito, and
T Teramoto, and
H Kato, and
M Kinoshita, and
M Kawamura, and
H Hayashi, and
H Oka
January 1980,
Prostaglandins,
Y Hashimoto, and
C Naito, and
T Teramoto, and
H Kato, and
M Kinoshita, and
M Kawamura, and
H Hayashi, and
H Oka
May 1984,
Biochimica et biophysica acta,
Y Hashimoto, and
C Naito, and
T Teramoto, and
H Kato, and
M Kinoshita, and
M Kawamura, and
H Hayashi, and
H Oka
June 1985,
The Journal of investigative dermatology,
Y Hashimoto, and
C Naito, and
T Teramoto, and
H Kato, and
M Kinoshita, and
M Kawamura, and
H Hayashi, and
H Oka
January 1983,
Advances in prostaglandin, thromboxane, and leukotriene research,
Y Hashimoto, and
C Naito, and
T Teramoto, and
H Kato, and
M Kinoshita, and
M Kawamura, and
H Hayashi, and
H Oka
January 1987,
Advances in prostaglandin, thromboxane, and leukotriene research,
Y Hashimoto, and
C Naito, and
T Teramoto, and
H Kato, and
M Kinoshita, and
M Kawamura, and
H Hayashi, and
H Oka
May 1979,
Biochimica et biophysica acta,
Y Hashimoto, and
C Naito, and
T Teramoto, and
H Kato, and
M Kinoshita, and
M Kawamura, and
H Hayashi, and
H Oka
January 1992,
Archives of biochemistry and biophysics,
Y Hashimoto, and
C Naito, and
T Teramoto, and
H Kato, and
M Kinoshita, and
M Kawamura, and
H Hayashi, and
H Oka
April 1993,
Journal of lipid research,
Y Hashimoto, and
C Naito, and
T Teramoto, and
H Kato, and
M Kinoshita, and
M Kawamura, and
H Hayashi, and
H Oka
March 1992,
Journal of chromatography,
