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Rat placentas from fetuses of 18 and 20 days of gestation were collected, extracted, and examined for their capacity to stimulate GH release in vitro. The crude extract stimulated, in a dose-dependent fashion, GH release by rat anterior pituitary cells in monolayer culture. The biological and immunological activities retained on antirat GH-releasing factor immunoaffinity columns eluted on Sephadex G-75 (fine) columns with an estimated mol wt of 5000 daltons. Reverse phase HPLC of this material revealed the presence of two forms of GRF activity that eluted with retention times identical to those of synthetic rat GRF and its methionine sulfoxide counterpart [Met(O)27]GRF. The results demonstrate the presence of an immunoreactive and biologically active GRF in the rat placenta that is indistinguishable from rat hypothalamic GRF.
A Baird, and
W B Wehrenberg, and
P Böhlen, and
N Ling
August 1988,
Endocrinology,
A Baird, and
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January 1990,
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A Baird, and
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January 1997,
Biology of the neonate,
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December 1994,
Biology of reproduction,
A Baird, and
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June 1984,
The Journal of clinical endocrinology and metabolism,
A Baird, and
W B Wehrenberg, and
P Böhlen, and
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June 1987,
Neuroscience letters,
A Baird, and
W B Wehrenberg, and
P Böhlen, and
N Ling
January 1984,
Nature,
A Baird, and
W B Wehrenberg, and
P Böhlen, and
N Ling
January 1990,
Life sciences,
A Baird, and
W B Wehrenberg, and
P Böhlen, and
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Cancer,
A Baird, and
W B Wehrenberg, and
P Böhlen, and
N Ling
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Reproduction, fertility, and development,
