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Long-term insulin secretion was investigated in seven biohybrid capillary devices seeded with canine islets. Approximately 50,000 islets could be isolated from a single canine pancreas using collagenase digestion in conjunction with the recently described Velcro technique. Devices were perfused with tissue culture medium 199 containing 300 mg/dl glucose. Insulin secretion fell during the first 1-2 days of culture from approximately 18 to 6 U/day. After 3-4 wk of perfusion, however, there was a gradual rise in insulin output that reached greater than 15 U/day after 7 wk. Insulin output eventually stabilized at 18-20 U/day. Two of these devices were studied for 80 and 86 days, respectively, and continued to secrete these same amounts of insulin. Glucose-induced insulin release was studied after 1 and 4 wk of culture and was well preserved.
Y Araki, and
B A Solomon, and
R M Basile, and
W L Chick
January 1994,
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Y Araki, and
B A Solomon, and
R M Basile, and
W L Chick
May 1991,
Science (New York, N.Y.),
Y Araki, and
B A Solomon, and
R M Basile, and
W L Chick
April 1992,
Transplantation proceedings,
Y Araki, and
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W L Chick
November 1993,
Transplantation,
Y Araki, and
B A Solomon, and
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W L Chick
December 1988,
Transplantation,
Y Araki, and
B A Solomon, and
R M Basile, and
W L Chick
January 2001,
Journal of drug targeting,
Y Araki, and
B A Solomon, and
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W L Chick
December 1985,
Nihon rinsho. Japanese journal of clinical medicine,
Y Araki, and
B A Solomon, and
R M Basile, and
W L Chick
February 1999,
Advanced drug delivery reviews,
Y Araki, and
B A Solomon, and
R M Basile, and
W L Chick
September 1997,
FEBS letters,
Y Araki, and
B A Solomon, and
R M Basile, and
W L Chick
April 1979,
Surgery, gynecology & obstetrics,
