Biomaterial Database

[An experimental study on pyogenic osteomyelitis with special reference to polymicrobial infections]. 1985

S Hidaka

The author used the following method to successfully devise a model of experimental osteomyelitis caused by two different bacteria. A 3-mm silk thread (No. 5) soaked in Staphylococcus aureus (1.0 X 10(4) CFU) and Pseudomonas aeruginosa (1.0 X 10(5) CFU) suspensions was dried under reduced pressure. The silk thread was inserted into the metaphysis of the right tibia of mice. Experimental osteomyelitis caused by these two species of bacteria was successfully produced in all the mice, and this infection was similar to osteomyelitis in man. After prior infection with S. aureus, P. aeruginosa (1.0 X 10(3) CFU) was inoculated directly into the tibia of mice during the acute and chronic stages of the S. aureus infection, and secondary infection by P. aeruginosa was confirmed in all the mice. Using this experimental model of osteomyelitis, the author administered an antibiotic that is effective against only S. aureus in mice and observed abrupt growth of P. aeruginosa to elucidate certain aspects of the bacterial replacement phenomena.

UI	MeSH Term	Description	Entries
D008813	Mice, Inbred ICR	An inbred strain of mouse that is used as a general purpose research strain, for therapeutic drug testing, and for the genetic analysis of CARCINOGEN-induced COLON CANCER.	Mice, Inbred ICRC,Mice, ICR,Mouse, ICR,Mouse, Inbred ICR,Mouse, Inbred ICRC,ICR Mice,ICR Mice, Inbred,ICR Mouse,ICR Mouse, Inbred,ICRC Mice, Inbred,ICRC Mouse, Inbred,Inbred ICR Mice,Inbred ICR Mouse,Inbred ICRC Mice,Inbred ICRC Mouse
D010019	Osteomyelitis	INFLAMMATION of the bone as a result of infection. It may be caused by a variety of infectious agents, especially pyogenic (PUS - producing) BACTERIA.	Osteomyelitides
D011550	Pseudomonas aeruginosa	A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.	Bacillus aeruginosus,Bacillus pyocyaneus,Bacterium aeruginosum,Bacterium pyocyaneum,Micrococcus pyocyaneus,Pseudomonas polycolor,Pseudomonas pyocyanea
D011552	Pseudomonas Infections	Infections with bacteria of the genus PSEUDOMONAS.	Infections, Pseudomonas,Pseudomonas aeruginosa Infection,Infection, Pseudomonas,Pseudomonas Infection,Pseudomonas aeruginosa Infections
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013203	Staphylococcal Infections	Infections with bacteria of the genus STAPHYLOCOCCUS.	Infections, Staphylococcal,Staphylococcus aureus Infection,Staphylococcal Infection,Staphylococcus aureus Infections
D013211	Staphylococcus aureus	Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
D013492	Suppuration	A pathologic process consisting in the formation of pus.	Pus
D013977	Tibia	The second longest bone of the skeleton. It is located on the medial side of the lower leg, articulating with the FIBULA laterally, the TALUS distally, and the FEMUR proximally.	Tibias