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Differential distribution of carboxypeptidase-processing enzyme activity and immunoreactivity in membrane and soluble components of chromaffin granules. 1985

V Y Hook

A comparison of carboxypeptidase-processing enzyme activity and immunoreactivity showed that they were differentially distributed in soluble and membrane components of bovine adrenal medulla chromaffin granules. The majority of enzyme activity (80% of total activity in the granules) was present in the soluble fraction, but the number of enzyme molecules was distributed equally between the soluble and membrane fractions. When equivalent amounts of carboxypeptidase enzyme (ng of immunoreactivity) in each fraction were compared, the carboxypeptidase in the soluble component appeared to be five to six times more active than the membrane-bound form of the enzyme. The soluble and membrane components of the granules may represent populations of enzyme at different states of activation. This finding could have important implications for the regulation of the carboxypeptidase-processing enzyme.

UI MeSH Term Description Entries
D002268 Carboxypeptidases Enzymes that act at a free C-terminus of a polypeptide to liberate a single amino acid residue. Carboxypeptidase
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D002837 Chromaffin Granules Organelles in CHROMAFFIN CELLS located in the adrenal glands and various other organs. These granules are the site of the synthesis, storage, metabolism, and secretion of EPINEPHRINE and NOREPINEPHRINE. Chromaffin Granule,Granule, Chromaffin
D002838 Chromaffin System The cells of the body which stain with chromium salts. They occur along the sympathetic nerves, in the adrenal gland, and in various other organs. Argentaffin System,Argentaffin Systems,Chromaffin Systems,System, Argentaffin,System, Chromaffin,Systems, Argentaffin,Systems, Chromaffin
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013347 Subcellular Fractions Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163) Fraction, Subcellular,Fractions, Subcellular,Subcellular Fraction
D043423 Carboxypeptidase H A ZINC-containing exopeptidase primarily found in SECRETORY VESICLES of endocrine and neuroendocrine cells. It catalyzes the cleavage of C-terminal ARGININE or LYSINE residues from polypeptides and is active in processing precursors of PEPTIDE HORMONES and other bioactive peptides. Carboxypeptidase E,Enkephalin Convertase,Enkephalin-Forming Carboxypeptidase,Enkephalin-Synthesizing Carboxypeptidase,Carboxypeptidase, Enkephalin-Forming,Carboxypeptidase, Enkephalin-Synthesizing,Convertase, Enkephalin,Enkephalin Forming Carboxypeptidase,Enkephalin Synthesizing Carboxypeptidase

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