Biomaterial Database

An improved cytochemical method for the evaluation of eosinophil colonies in soft-agar cultures from human blood or bone marrow. 1985

J Dufer

A simple peroxidase staining method specific for eosinophils using 4-chloro-1-naphthol as substrate and 1,2,4-benzene triol as selective inhibitor is applied to the analysis of eosinophil colonies grown in soft-agar cultures form human blood or bone marrow. Eosinophil colonies appear strongly stained in black whereas other types of colonies (neutrophils and macrophages) remain colourless. The proposed method is easy to be performed, quick, reliable, may be associated without major problems to many other cytochemical stains and promises to be of value in hemopoiesis studies.

UI	MeSH Term	Description	Entries
D010544	Peroxidases		Ovoperoxidase
D001853	Bone Marrow	The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.	Marrow,Red Marrow,Yellow Marrow,Marrow, Bone,Marrow, Red,Marrow, Yellow
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D004804	Eosinophils	Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.	Eosinophil
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults
D000362	Agar	A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.