BIOMAT Database Logo BIOMAT Database Logo

Evaluation of the measurement of B protein of plasma low density lipoprotein by radial immunodiffusion. 1985

A J Lutalo-Bosa, and J L Adolphson, and J J Albers

Radial immunodiffusion (RID) has been used for determination of low density lipoprotein (LDL) B protein in plasma. During measurement of B protein in plasma and the d less than and d greater than 1.019 g/ml plasma fractions by RID in 1.0%, 1.5%, 2.0%, and 2.5% agarose, the d less than 1.019 g/ml lipoproteins diffuse in the agarose and produce precipitin rings. Among normotriglyceridemic subjects, the B protein values in whole plasma obtained by RID using 1.5 to 2.5% agarose were only slightly higher than the values in the d greater than 1.019 g/ml fraction obtained by RID and closely approximated the values obtained in the d greater than 1.019 g/ml fraction by radioimmunoassay. However, among the hypertriglyceridemic subjects, the RID measurement of B protein in plasma using 1.0 to 2.5% agarose overestimated the LDL B protein levels in plasma. The RID procedure at agarose concentrations of 1.5% to 2.5% can be used to estimate plasma LDL B protein levels in normotriglyceridemic subjects. However, measurement of LDL B protein by RID in plasma of hypertriglyceridemic subjects must be interpreted with caution; the LDL B protein is overestimated by this procedure because of the contribution by the d less than 1.019 g/ml lipoproteins to the B protein value.

UI MeSH Term Description Entries
D008077 Lipoproteins, LDL A class of lipoproteins of small size (18-25 nm) and light (1.019-1.063 g/ml) particles with a core composed mainly of CHOLESTEROL ESTERS and smaller amounts of TRIGLYCERIDES. The surface monolayer consists mostly of PHOSPHOLIPIDS, a single copy of APOLIPOPROTEIN B-100, and free cholesterol molecules. The main LDL function is to transport cholesterol and cholesterol esters to extrahepatic tissues. Low-Density Lipoprotein,Low-Density Lipoproteins,beta-Lipoprotein,beta-Lipoproteins,LDL(1),LDL(2),LDL-1,LDL-2,LDL1,LDL2,Low-Density Lipoprotein 1,Low-Density Lipoprotein 2,LDL Lipoproteins,Lipoprotein, Low-Density,Lipoproteins, Low-Density,Low Density Lipoprotein,Low Density Lipoprotein 1,Low Density Lipoprotein 2,Low Density Lipoproteins,beta Lipoprotein,beta Lipoproteins
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D005779 Immunodiffusion Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction. Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001055 Apolipoproteins B Major structural proteins of triacylglycerol-rich LIPOPROTEINS. There are two forms, apolipoprotein B-100 and apolipoprotein B-48, both derived from a single gene. ApoB-100 expressed in the liver is found in low-density lipoproteins (LIPOPROTEINS, LDL; LIPOPROTEINS, VLDL). ApoB-48 expressed in the intestine is found in CHYLOMICRONS. They are important in the biosynthesis, transport, and metabolism of triacylglycerol-rich lipoproteins. Plasma Apo-B levels are high in atherosclerotic patients but non-detectable in ABETALIPOPROTEINEMIA. Apo-B,Apo B,ApoB,Apoprotein (B),Apoproteins B
D012685 Sepharose Agarose,Sepharose 4B,Sepharose C1 4B,4B, Sepharose C1,C1 4B, Sepharose
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures

Related Publications

A J Lutalo-Bosa, and J L Adolphson, and J J Albers
Low-density-lipoprotein apoprotein B in plasma as measured by radial immunodiffusion and rocket immunoelectrophoresis.
November 1981, Clinical chemistry,
A J Lutalo-Bosa, and J L Adolphson, and J J Albers
Measurement of apolipoprotein B by radial immunodiffusion.
May 1983, Clinical chemistry,
A J Lutalo-Bosa, and J L Adolphson, and J J Albers
[The measurement of apolipoprotein A-I and A-II levels of human plasma high density lipoprotein (HDL) by single radial immunodiffusion (SRID) method].
November 1982, Nihon Ronen Igakkai zasshi. Japanese journal of geriatrics,
A J Lutalo-Bosa, and J L Adolphson, and J J Albers
Determination of B protein of low density lipoprotein directly in plasma.
November 1975, Journal of lipid research,
A J Lutalo-Bosa, and J L Adolphson, and J J Albers
Evaluation of Malondialdehyde Low-Density Lipoprotein Stratified by Low-Density Lipoprotein Cholesterol.
July 2017, Clinical laboratory,
A J Lutalo-Bosa, and J L Adolphson, and J J Albers
[Determination of plasma proteins by radial immunodiffusion].
January 1971, Zeitschrift fur medizinische Labortechnik,
A J Lutalo-Bosa, and J L Adolphson, and J J Albers
Reliability of low-density lipoprotein cholesterol, non-high-density lipoprotein cholesterol, and apolipoprotein B measurement.
January 2011, Journal of clinical lipidology,
A J Lutalo-Bosa, and J L Adolphson, and J J Albers
The turnover of human plasma very low density lipoprotein protein.
December 1977, Biochimica et biophysica acta,
A J Lutalo-Bosa, and J L Adolphson, and J J Albers
Evaluation of methods for the measurement of low-density lipoprotein cholesterol.
March 2005, Journal of cardiovascular pharmacology and therapeutics,
A J Lutalo-Bosa, and J L Adolphson, and J J Albers
Principles of protein estimation by radial immunodiffusion.
February 1971, Journal of clinical pathology,
Copied contents to your clipboard!