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Fibrinolytic activity in porcine follicular fluid and plasminogen activator production by porcine granulosa cells in response to gonadotropins is described. Fibrinolytic activity was determined using a solid phase assay in which 125I-fibrinogen was coupled to latex beads. Urokinase plus plasminogen served as the standard. Porcine follicular fluid contained protease inhibitors as evidenced by its ability to inhibit the activity of an urokinase-plasminogen standard. Removal of these inhibitors by acid precipitation revealed plasminogen-dependent and -independent fibrinolytic activity. Cultured granulosa cells produced plasminogen activator in amounts that increased over time. This pattern was most significant in cells stimulated by hCG. Cells incubated with hCG produced significantly more plasminogen activator when compared to those cells cultured in absence of gonadotropin. The enhancement was most marked during the second 48 hrs in culture (p = 0.032). FSH was found to have no stimulatory effect on plasminogen activator production by cultured porcine granulosa cells.
K J Shaw, and
J D Campeau, and
P C Roche, and
G S diZerega
April 1989,
Endocrinology,
K J Shaw, and
J D Campeau, and
P C Roche, and
G S diZerega
April 1983,
Endocrinology,
K J Shaw, and
J D Campeau, and
P C Roche, and
G S diZerega
January 1986,
Endocrinology,
K J Shaw, and
J D Campeau, and
P C Roche, and
G S diZerega
April 1993,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
K J Shaw, and
J D Campeau, and
P C Roche, and
G S diZerega
January 1994,
The Journal of clinical endocrinology and metabolism,
K J Shaw, and
J D Campeau, and
P C Roche, and
G S diZerega
January 1995,
Developmental biology,
K J Shaw, and
J D Campeau, and
P C Roche, and
G S diZerega
May 1994,
Cellular and molecular biology (Noisy-le-Grand, France),
K J Shaw, and
J D Campeau, and
P C Roche, and
G S diZerega
November 1973,
Endocrinology,
K J Shaw, and
J D Campeau, and
P C Roche, and
G S diZerega
July 1972,
Endocrinology,
K J Shaw, and
J D Campeau, and
P C Roche, and
G S diZerega
September 1988,
Endocrinology,
