BIOMAT Database Logo BIOMAT Database Logo

Immunochemical determination of human apolipoprotein B by laser nephelometry. 1979

C Fievet-Desreumaux, and E Dedonder-Decoopman, and J C Fruchart, and P Dewailly, and G Sezille

The Hyland laser nephelometer PDQ system for the assay of apolipoprotein B (apo-B) in human serum is described. Within and between-batch precision, accuracy and reliability are discussed. This instrument represents an important development in the immunochemical assay of apo-B, and the speed, precision, and convenience of the methodology make such a system attractive. Quantitation of apo-B was assessed in normal and hyperlipaemic subjects. Comparisons were made with two other specific and sensitive immunological methods for quantifying apo-B: enzymeimmunoassay (EIA) and rocket immunoelectrophoresis (RIE). Results obtained by the three methods correlated very well.

UI MeSH Term Description Entries
D007120 Immunochemistry Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
D007122 Immunoelectrophoresis A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
D007124 Immunoenzyme Techniques Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens. Antibody Enzyme Technique, Unlabeled,Enzyme Immunoassay,Enzyme-Labeled Antibody Technique,Immunoassay, Enzyme,Immunoperoxidase Techniques,Peroxidase-Antiperoxidase Complex Technique,Peroxidase-Labeled Antibody Technique,Antibody Enzyme Technic, Unlabeled,Enzyme-Labeled Antibody Technic,Immunoenzyme Technics,Immunoperoxidase Technics,Peroxidase-Antiperoxidase Complex Technic,Peroxidase-Labeled Antibody Technic,Antibody Technic, Enzyme-Labeled,Antibody Technic, Peroxidase-Labeled,Antibody Technics, Enzyme-Labeled,Antibody Technics, Peroxidase-Labeled,Antibody Technique, Enzyme-Labeled,Antibody Technique, Peroxidase-Labeled,Antibody Techniques, Enzyme-Labeled,Antibody Techniques, Peroxidase-Labeled,Enzyme Immunoassays,Enzyme Labeled Antibody Technic,Enzyme Labeled Antibody Technique,Enzyme-Labeled Antibody Technics,Enzyme-Labeled Antibody Techniques,Immunoassays, Enzyme,Immunoenzyme Technic,Immunoenzyme Technique,Immunoperoxidase Technic,Immunoperoxidase Technique,Peroxidase Antiperoxidase Complex Technic,Peroxidase Antiperoxidase Complex Technique,Peroxidase Labeled Antibody Technic,Peroxidase Labeled Antibody Technique,Peroxidase-Antiperoxidase Complex Technics,Peroxidase-Antiperoxidase Complex Techniques,Peroxidase-Labeled Antibody Technics,Peroxidase-Labeled Antibody Techniques,Technic, Enzyme-Labeled Antibody,Technic, Immunoenzyme,Technic, Immunoperoxidase,Technic, Peroxidase-Antiperoxidase Complex,Technic, Peroxidase-Labeled Antibody,Technics, Enzyme-Labeled Antibody,Technics, Immunoenzyme,Technics, Immunoperoxidase,Technics, Peroxidase-Antiperoxidase Complex,Technics, Peroxidase-Labeled Antibody,Technique, Enzyme-Labeled Antibody,Technique, Immunoenzyme,Technique, Immunoperoxidase,Technique, Peroxidase-Antiperoxidase Complex,Technique, Peroxidase-Labeled Antibody,Techniques, Enzyme-Labeled Antibody,Techniques, Immunoenzyme,Techniques, Immunoperoxidase,Techniques, Peroxidase-Antiperoxidase Complex,Techniques, Peroxidase-Labeled Antibody
D007834 Lasers An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum. Masers,Continuous Wave Lasers,Pulsed Lasers,Q-Switched Lasers,Continuous Wave Laser,Laser,Laser, Continuous Wave,Laser, Pulsed,Laser, Q-Switched,Lasers, Continuous Wave,Lasers, Pulsed,Lasers, Q-Switched,Maser,Pulsed Laser,Q Switched Lasers,Q-Switched Laser
D009391 Nephelometry and Turbidimetry Chemical analysis based on the phenomenon whereby light, passing through a medium with dispersed particles of a different refractive index from that of the medium, is attenuated in intensity by scattering. In turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured. In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam. Turbidimetry,Nephelometry,Turbidimetry and Nephelometry
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001053 Apolipoproteins Protein components on the surface of LIPOPROTEINS. They form a layer surrounding the hydrophobic lipid core. There are several classes of apolipoproteins with each playing a different role in lipid transport and LIPID METABOLISM. These proteins are synthesized mainly in the LIVER and the INTESTINES. Apolipoprotein

Related Publications

C Fievet-Desreumaux, and E Dedonder-Decoopman, and J C Fruchart, and P Dewailly, and G Sezille
Immunochemical determination of human lysozyme by laser nephelometry.
June 1977, Clinical chemistry,
C Fievet-Desreumaux, and E Dedonder-Decoopman, and J C Fruchart, and P Dewailly, and G Sezille
[Quantitative determination of apolipoprotein A in human serum by laser nephelometry].
January 1981, La Ricerca in clinica e in laboratorio,
C Fievet-Desreumaux, and E Dedonder-Decoopman, and J C Fruchart, and P Dewailly, and G Sezille
Determination of apolipoprotein B by kinetic (rate) nephelometry.
August 1982, Journal of lipid research,
C Fievet-Desreumaux, and E Dedonder-Decoopman, and J C Fruchart, and P Dewailly, and G Sezille
Determination of human apolipoproteins A-I, B, and E by laser nephelometry.
January 1984, Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
C Fievet-Desreumaux, and E Dedonder-Decoopman, and J C Fruchart, and P Dewailly, and G Sezille
Nephelometry of apolipoprotein B in human serum.
February 1979, Clinical chemistry,
C Fievet-Desreumaux, and E Dedonder-Decoopman, and J C Fruchart, and P Dewailly, and G Sezille
Determination of apolipoprotein A-I by kinetic nephelometry.
January 1981, Biochimica et biophysica acta,
C Fievet-Desreumaux, and E Dedonder-Decoopman, and J C Fruchart, and P Dewailly, and G Sezille
[Protein determination by means of laser nephelometry].
January 1977, La Ricerca in clinica e in laboratorio,
C Fievet-Desreumaux, and E Dedonder-Decoopman, and J C Fruchart, and P Dewailly, and G Sezille
[Determination of coagulofibrinolytic factors by laser nephelometry (author's transl)].
January 1981, Rinsho byori. The Japanese journal of clinical pathology,
C Fievet-Desreumaux, and E Dedonder-Decoopman, and J C Fruchart, and P Dewailly, and G Sezille
[Determination of immunoglobulins by laser nephelometry using monospecific antisera].
January 1984, Laboratornoe delo,
C Fievet-Desreumaux, and E Dedonder-Decoopman, and J C Fruchart, and P Dewailly, and G Sezille
[Determination of apolipoproteins A-I and B using a laser nephelometry method].
November 1983, Vnitrni lekarstvi,
Copied contents to your clipboard!