-transparent.png){kind=logo}
Human peripheral blood mononuclear lymphocytes produce interferon gamma (IFN-gamma) in response to stimulation by mitogens. Previous studies on the kinetics of IFN-gamma mRNA production upon mitogen induction, showed that steady-state levels of mRNA increased to a maximum at 12-24 h post-induction after which they declined to levels not detectable by the assay used. We show here that in mitogen induced peripheral blood lymphocytes, inhibition of protein synthesis using three different inhibitors (cycloheximide, puromycin, pactamycin) resulted in an increase in the steady-state levels of IFN-gamma mRNA. The levels of mRNA in cells treated with inhibitor 16 h post-induction were up to 3-fold higher than in untreated cells. Superinduction was possible up to 40 h post-induction after which the steady-state levels of mRNA had declined to limits below detection; IFN-gamma mRNA was not superinduced by cycloheximide in the presence of actinomycin D.
K W Siggens, and
A G Morris
June 1981,
Proceedings of the National Academy of Sciences of the United States of America,
K W Siggens, and
A G Morris
August 1978,
Virology,
K W Siggens, and
A G Morris
January 1980,
Medycyna doswiadczalna i mikrobiologia,
K W Siggens, and
A G Morris
January 1981,
Postepy higieny i medycyny doswiadczalnej,
K W Siggens, and
A G Morris
May 1987,
Molecular immunology,
K W Siggens, and
A G Morris
March 1987,
The EMBO journal,
K W Siggens, and
A G Morris
October 1971,
Journal of the National Cancer Institute,
K W Siggens, and
A G Morris
February 1991,
Transplantation proceedings,
K W Siggens, and
A G Morris
March 1987,
The EMBO journal,
