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Polyclonal rheumatoid factor (pRF) was isolated from sera seropositive for RF and it was radioiodinated with lactoperoxidase method. Using the 125I-pRF and IgG-p-azobenzamidoethyl Sepharose 6B, a competitive inhibition radioassay for detecting immune complexes is described. The assay can be performed in 90 min utilizing 10 microliter of serum with good reproducibility and endogenous RF in test serum did not interfere the reaction. The test can detect complexes nearly to 8s and as low as 0.1 microgram/ml of aggregated human IgG. Decomplementation by heating test sera is unnecessary. The assay was performed at 4 degrees C but less inhibition activity was obtained when it was carried out at 24 degrees C.
J Narumoto, and
T Arima, and
K Kunishi, and
T Yasuhara, and
K Suwaki, and
H Shimomura, and
H Nagashima
January 1981,
Gastroenterologia Japonica,
J Narumoto, and
T Arima, and
K Kunishi, and
T Yasuhara, and
K Suwaki, and
H Shimomura, and
H Nagashima
June 2003,
Immunology,
J Narumoto, and
T Arima, and
K Kunishi, and
T Yasuhara, and
K Suwaki, and
H Shimomura, and
H Nagashima
January 1979,
Journal of immunological methods,
J Narumoto, and
T Arima, and
K Kunishi, and
T Yasuhara, and
K Suwaki, and
H Shimomura, and
H Nagashima
January 1981,
La Revue de medecine interne,
J Narumoto, and
T Arima, and
K Kunishi, and
T Yasuhara, and
K Suwaki, and
H Shimomura, and
H Nagashima
January 1981,
Methods in enzymology,
J Narumoto, and
T Arima, and
K Kunishi, and
T Yasuhara, and
K Suwaki, and
H Shimomura, and
H Nagashima
January 1993,
Clinical and experimental rheumatology,
J Narumoto, and
T Arima, and
K Kunishi, and
T Yasuhara, and
K Suwaki, and
H Shimomura, and
H Nagashima
May 2000,
Annals of the rheumatic diseases,
J Narumoto, and
T Arima, and
K Kunishi, and
T Yasuhara, and
K Suwaki, and
H Shimomura, and
H Nagashima
January 1979,
Journal of immunological methods,
J Narumoto, and
T Arima, and
K Kunishi, and
T Yasuhara, and
K Suwaki, and
H Shimomura, and
H Nagashima
December 1983,
Journal of immunological methods,
J Narumoto, and
T Arima, and
K Kunishi, and
T Yasuhara, and
K Suwaki, and
H Shimomura, and
H Nagashima
January 1980,
Journal of immunological methods,
