BIOMAT Database Logo BIOMAT Database Logo

Propagation of Toxoplasma gondii in suspension cultures of HeLa cells. 1979

A Valkoun, and J Cinátl

A novel method of Toxoplasma gondii cultivation in suspension cultures has been introduced using silicone-coated glass vessels (working volume 100 ml). The cells were kept in suspension by a magnetic impeller at 75 rpm at a temperature of 37 degrees C. HeLa cells grown on MEM with calf serum were used as host substrate. The HeLa cells were infected with zoites of Toxoplasma gondii virulent P strain. After seven days the host cells were destroyed by the toxoplasmas and the number of zoites was up to 24 x 10(6)/ml.

UI MeSH Term Description Entries
D008828 Microbiological Techniques Techniques used in microbiology. Microbiologic Technic,Microbiologic Technics,Microbiologic Technique,Microbiological Technics,Technic, Microbiologic,Technics, Microbiological,Technique, Microbiologic,Techniques, Microbiologic,Microbiologic Techniques,Microbiological Technic,Microbiological Technique,Technic, Microbiological,Technics, Microbiologic,Technique, Microbiological,Techniques, Microbiological
D006367 HeLa Cells The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays. Cell, HeLa,Cells, HeLa,HeLa Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014122 Toxoplasma A genus of protozoa parasitic to birds and mammals. T. gondii is one of the most common infectious pathogenic animal parasites of man. Toxoplasma gondii,Toxoplasma gondius,Toxoplasmas,gondius, Toxoplasma

Related Publications

A Valkoun, and J Cinátl
[Long-term cultures of Toxoplasma gondii in HeLa cells].
June 1957, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. 1. Abt. Medizinisch-hygienische Bakteriologie, Virusforschung und Parasitologie. Originale,
A Valkoun, and J Cinátl
[Long-term tissue cultures of Toxoplasma gondii on HeLa cells].
January 1957, Ceskoslovenska epidemiologie, mikrobiologie, imunologie,
A Valkoun, and J Cinátl
Continual cultivation of Toxoplasma gondii on HeLa cells.
January 1983, Folia parasitologica,
A Valkoun, and J Cinátl
Rhinovirus replication in suspension cultures of HeLa cells.
January 1970, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
A Valkoun, and J Cinátl
Long-term culture of Toxoplasma gondii on HeLa cells.
January 1960, Journal of hygiene, epidemiology, microbiology, and immunology,
A Valkoun, and J Cinátl
Gefitinib inhibits the growth of Toxoplasma gondii in HeLa cells.
August 2014, The Korean journal of parasitology,
A Valkoun, and J Cinátl
Toxoplasma gondii propagation in cell cultures and preservation at liquid nitrogen temperatures.
January 1969, Cryobiology,
A Valkoun, and J Cinátl
Testosterone Augments Propagation of Toxoplasma gondii in Glioblastoma Cells In Vitro.
September 2022, Acta parasitologica,
A Valkoun, and J Cinátl
Long-term in vitro suspension culture of Toxoplasma and HeLa cells.
May 1987, Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology,
A Valkoun, and J Cinátl
The propagation of tubercle bacilli in cultures of HeLa cells.
April 1956, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. 1. Abt. Medizinisch-hygienische Bakteriologie, Virusforschung und Parasitologie. Originale,
Copied contents to your clipboard!