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Human eosinophils with a density of over 1.095 were isolated from peripheral blood by dextran sedimentation, centrifugation with Lymphoprep and density gradients with Percoll. After the cells (1 X 10(5)/ml) were incubated with 1 microgram/ml calcium ionophore A23187 for 20 min, leukotriene C4 (LTC4) content in the supernatant was measured by radioimmunoassay. The generation of LTC4 was significantly higher in the cells from extrinsic asthmatics (23.5 +/- 14.8 ng/10(6) cells, mean +/- SD, n = 26, P less than 0.01) and intrinsic asthmatics (24.6 +/- 20.6 ng/10(6) cells, n = 27, P less than 0.01 as compared with normal healthy subjects (8.3 +/- 7.7 ng/10(6) cells, n = 10). There was no significant difference in the generation of LTC4 between intrinsic and extrinsic asthmatics. These observations indicate that eosinophils from asthmatic patients have increased ability to release LTC4.
N Taniguchi, and
H Mita, and
H Saito, and
Y Yui, and
T Kajita, and
T Shida
January 1987,
Annales de l'Institut Pasteur. Immunology,
N Taniguchi, and
H Mita, and
H Saito, and
Y Yui, and
T Kajita, and
T Shida
October 1988,
The American review of respiratory disease,
N Taniguchi, and
H Mita, and
H Saito, and
Y Yui, and
T Kajita, and
T Shida
June 1984,
Clinical and experimental immunology,
N Taniguchi, and
H Mita, and
H Saito, and
Y Yui, and
T Kajita, and
T Shida
February 1996,
Thorax,
N Taniguchi, and
H Mita, and
H Saito, and
Y Yui, and
T Kajita, and
T Shida
September 1995,
Biochemical and biophysical research communications,
N Taniguchi, and
H Mita, and
H Saito, and
Y Yui, and
T Kajita, and
T Shida
May 1986,
Prostaglandins,
N Taniguchi, and
H Mita, and
H Saito, and
Y Yui, and
T Kajita, and
T Shida
December 1994,
Archives of disease in childhood,
N Taniguchi, and
H Mita, and
H Saito, and
Y Yui, and
T Kajita, and
T Shida
October 1988,
The Journal of allergy and clinical immunology,
N Taniguchi, and
H Mita, and
H Saito, and
Y Yui, and
T Kajita, and
T Shida
February 1982,
The Journal of experimental medicine,
N Taniguchi, and
H Mita, and
H Saito, and
Y Yui, and
T Kajita, and
T Shida
September 1990,
Annals of allergy,
