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Alkaline phosphatase was purified from bovine polymorphonuclear neutrophils by butanol extraction and a combination of ion exchange, gel filtration and affinity chromatography. The enzyme was partially purified 2300-fold with a 4.7% yield and a sp. act. of 206 units/mg of protein. Polyacrylamide gel electrophoresis in sodium dodecyl sulfate indicated a single activity band with the mol. wt of 165,000. The pH optima for the enzyme were 10.0 with p-nitrophenylphosphate and phenylphosphate and were 9.0 when beta-glycerophosphate, AMP and ADP were used. The enzyme was activated by Mg2+, Mn2+, Co2+ and Ni2+ but was inhibited by Zn2+. The enzyme was inhibited by EDTA and the EDTA-inactivated enzyme was reactivated by Mg2+, Mn2+ and Co2+ but not Zn2+.
S Yasuura, and
I Nagaoka, and
T Yamashita, and
T Namihisa
July 1968,
Enzymologia,
S Yasuura, and
I Nagaoka, and
T Yamashita, and
T Namihisa
June 1976,
Biochimica et biophysica acta,
S Yasuura, and
I Nagaoka, and
T Yamashita, and
T Namihisa
January 1985,
The International journal of biochemistry,
S Yasuura, and
I Nagaoka, and
T Yamashita, and
T Namihisa
December 1952,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
S Yasuura, and
I Nagaoka, and
T Yamashita, and
T Namihisa
January 1979,
The International journal of biochemistry,
S Yasuura, and
I Nagaoka, and
T Yamashita, and
T Namihisa
September 1985,
Archives of biochemistry and biophysics,
S Yasuura, and
I Nagaoka, and
T Yamashita, and
T Namihisa
December 1985,
Biochemistry,
S Yasuura, and
I Nagaoka, and
T Yamashita, and
T Namihisa
November 1987,
Biochemical and biophysical research communications,
S Yasuura, and
I Nagaoka, and
T Yamashita, and
T Namihisa
January 1982,
Archives of oral biology,
S Yasuura, and
I Nagaoka, and
T Yamashita, and
T Namihisa
January 1966,
The Journal of biological chemistry,
