Biomaterial Database

[Immunologic identification of Streptococcus mutans using microimmunodiffusion and counterimmunoelectrophoresis technics]. 1985

M B Borgarelli, and R Marfort, and C A Sabelli, and M Marcantoni, and M T Mateo

Cátedra de Microbiología y Parasitología, Facultad de Odontología, Universidad de Buenos Aires, Argentina.

The St. mutans strains studied were: HS6 "a"; BHT "b"; IB "c"; B13 "d"; LM7 "e"; OMZ175 "f"; OMZ65 "g"; SL1 "SL"; 6715 "d/g". Immune sera and antigen preparation as well as antigen-antibody reactions were performed using the above-mentioned strains. The immune sera were obtained following Knox technique, including formolated immunogen with and without adjuvant. Linzer method was followed for rabbit immunization. The antigen extract were prepared according to Rantz and Randall method. The antigen-antibody reaction were carried out by microimmunodiffusion (MID) Ouchterlony technique and by counterimmunoelectrophoresis (CIE) according to De Torres method. A good correlation was found in the antigen-antibody reactions for the serotypes studied. MID and CIE revealed some cross-reactions, as already described by many investigators. The results obtained are compatible with St. mutans antigenic instability. The eight serotypes from St. mutans could possibly be reduced to four related groups: Group 1: serotypes "a-d-g-SL"; Group 2: serotype "e-f"; Group 3: serotype "c" (uncertain position: it can be related with group 1 or 2); Group 4: serotype "b". CIE is a convenient technique to be incorporated for immunological determination.

UI	MeSH Term	Description	Entries
D007122	Immunoelectrophoresis	A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
D003378	Counterimmunoelectrophoresis	Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow.	Electrosyneresis,Immunoelectroosmophoresis,Immunoelectrophoresis, Countercurrent,Immunoelectrophoresis, Crossover,Countercurrent Immunoelectrophoresis,Crossover Immunoelectrophoresis
D005779	Immunodiffusion	Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.	Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D000907	Antibodies, Bacterial	Immunoglobulins produced in a response to BACTERIAL ANTIGENS.	Bacterial Antibodies
D000937	Antigen-Antibody Reactions	The processes triggered by interactions of ANTIBODIES with their ANTIGENS.	Antigen Antibody Reactions,Antigen-Antibody Reaction,Reaction, Antigen-Antibody,Reactions, Antigen-Antibody
D000942	Antigens, Bacterial	Substances elaborated by bacteria that have antigenic activity.	Bacterial Antigen,Bacterial Antigens,Antigen, Bacterial
D013295	Streptococcus mutans	A polysaccharide-producing species of STREPTOCOCCUS isolated from human dental plaque.