Biomaterial Database

Value of the L-pyrrolidonyl-beta-naphthylamide hydrolysis test for identification of select gram-positive cocci. 1986

T R Oberhofer

A 4 hr test for hydrolysis of L-pyrrolidonyl-beta-naphthylamide (PYR) was evaluated for its use in identifying group A beta-hemolytic streptococci, group D enterococci, and Staphylococcus haemolyticus. All of the group A streptococci and the enterococci tested were positive in the PYR test, whereas all of the nongroup A, B, or D beta-hemolytic streptococci, all of the viridans, and all of the Streptococcus pneumoniae isolates tested were PYR negative. Two strains of Aerococcus species were PYR positive and could not be differentiated from enterococci in this test. All strains of S. haemolyticus, a single strain of S. intermedius and two of seven strains of S. warneri were PYR positive whereas all other staphylococci tested were PYR negative. The PYR test is suitable for routine, presumptive identification of group A streptococci, group D enterococci and S. haemolyticus in the clinical laboratory.

UI	MeSH Term	Description	Entries
D011760	Pyrrolidinones	A group of compounds that are derivatives of oxo-pyrrolidines. A member of this group is 2-oxo pyrrolidine, which is an intermediate in the manufacture of polyvinylpyrrolidone. (From Merck Index, 11th ed)	Pyrrolidinone,Pyrrolidone,Pyrrolidones
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006868	Hydrolysis	The process of cleaving a chemical compound by the addition of a molecule of water.
D013291	Streptococcus	A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.