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The biosynthesis of phospholipids during differentiation of normal and dystrophic muscle cells was studied using [32Pi] added to primary culture media. Labeled phosphatides were separated by two-dimensional thin-layer chromatography, exposing the plates to HCl vapors between solvents to cleave the alk-1-enyl bonds. Phosphatidylethanolamine labeling did not vary during myotube formation. Although lower labeling of phosphatidylcholine was observed in dystrophic myoblasts at the single cell stage, no differences were observed between normal and dystrophic cultures after cell contact and myotube formation. A sharp decrease in phosphoinositide content was observed as myoblasts first achieved cell contact and continued to decline slowly as myotubes developed. During myogenesis, phosphatidylcholine content gradually increased, from 18.4% of the total labeled phospholipid in dividing myoblasts to 48.6% of the labeled phospholipids in fully differentiated myotubes.
K C Leskawa, and
R E Erwin, and
E L Hogan
November 1986,
Experimental neurology,
K C Leskawa, and
R E Erwin, and
E L Hogan
January 1982,
Muscle & nerve,
K C Leskawa, and
R E Erwin, and
E L Hogan
December 1977,
Experimental neurology,
K C Leskawa, and
R E Erwin, and
E L Hogan
April 1971,
Journal of the neurological sciences,
K C Leskawa, and
R E Erwin, and
E L Hogan
March 1971,
Archives of neurology,
K C Leskawa, and
R E Erwin, and
E L Hogan
November 1960,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
K C Leskawa, and
R E Erwin, and
E L Hogan
February 1984,
The Journal of biological chemistry,
K C Leskawa, and
R E Erwin, and
E L Hogan
September 1990,
Cell calcium,
K C Leskawa, and
R E Erwin, and
E L Hogan
September 1986,
In vitro cellular & developmental biology : journal of the Tissue Culture Association,
K C Leskawa, and
R E Erwin, and
E L Hogan
January 1988,
General pharmacology,
