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Highly sensitive, effective and simple methods for determination of leukocytic interferons were developed. The methods are based on formation of immune complexes of interferon with anti-interferon monoclonal and polyclonal antibodies immobilized on an insoluble basis. As a result of interferon alpha 2 purification from the biomass of the interferon-producing bacteria the preparative amounts of the protein were obtained. These amounts were sufficient for the interferon structural and physico-chemical analysis. The isolated interferons are characterized by high stability on storage and may serve a basis for preparation of pharmaceutical agents.
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